Produksi Kitin Deasetilase Oleh Bacillus sp. SK II-5

Atmaja, Qintan Istighfarin (2015) Produksi Kitin Deasetilase Oleh Bacillus sp. SK II-5. Undergraduate thesis, Institut Technology Sepuluh Nopember.

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Abstract

Kitosan adalah produk deasetilasi kitin yang mempunyai banyak manfaat di berbagai bidang. Namun, selama ini solubilisasi kitin menjadi kitosan dilakukan melalui proses secara kimiawi yang tidak ramah lingkungan. Permasalahan dalam penelitian ini adalah bagaimana mengoptimalkan produksi kitin deasetilase sebagai agen biologi solubilisasi kitin oleh bakteri Bacillus sp. SK II-5 dengan memanfaatkan limbah perikanan. Pada penelitian ini dilakukan optimasi produksi kitin deasetilase pada suhu, pH dan medium produksi enzim. Kandungan protein diukur berdasarkan metode Bradford dengan BSA (Bovine Serum Albumin) sebagai standar. Serta dilakukan purifikasi enzim dengan metode presipitasi ammonium sulfat bertingkat kemudian protein kitin deasetilase dikarakterisasi dengan pengukuran terhadap titik isoelektrik, aktivitas enzim, kandungan protein dan elektroforesis SDS-PAGE. Hasil penelitian diperoleh aktivitas enzim paling optimum dihasilkan dari kombinasi medium kulit udang sebagai sumber karbon, pH 7 dan inkubasi pada suhu 60ºC dengan aktivitas enzim tertinggi hasil purifikasi amonium sulfat pada fraksi 60-75% sebesar 969,14 U/ml dengan kandungan protein 0,0024 mg/ml. Kitin deasetilase dari Bacillus sp. SK II-5 memiliki titik isoelektrik pada pH 5 dan berat molekul 45 kDa ===================================================================================================== Chitosan is a product of deacetylation chitin which has many benefits in various fields. Solubilization of chitin into chitosan has been done through chemical process which is harmful to the environment. The current study approaches the problem through green technology in which chitin deacetylase is optimized by Bacillus sp. SK II-5 as biological agent of chitin solubilization. The production optimization of chitin deacetylase conducted in this study include the temperature, pH and enriched medium. The protein content was measured by Bradford method with BSA (Bovine Serum Albumin) as standard. The enzyme was purified by ammonium sulfate precipitation and characterized by measuring the isoelectrical point, enzyme activity, protein content and molecular weight. Optimum enzyme activity was achieved through combination of medium shrimp shells as the carbon source with pH 7 at 60ºC. with the highest enzyme activity results of purification of ammonium sulfate at 60-75% fraction of 0.00528 U / ml with a protein content of 0.0024 mg / ml. Chitin deacetylase from Bacillus sp SK II-5 has an isoelectric point at pH 5 and molecular weight 45 kDa

Item Type: Thesis (Undergraduate)
Additional Information: RSBi 573.774 Atm p
Uncontrolled Keywords: Bacillus sp. SK II-5, Kitin Deasetilase, Karakterisasi Enzim, Optimasi Produksi
Subjects: Q Science > QH Biology > QH301 Biology
Divisions: Faculty of Mathematics and Science > Biology > (S1) Undergraduate Theses
Depositing User: Mr. Tondo Indra Nyata
Date Deposited: 19 Dec 2018 02:05
Last Modified: 19 Dec 2018 02:05
URI: http://repository.its.ac.id/id/eprint/60097

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