Widodo, Fajar Arif and Istiqfarin, - (2015) Pembuatan bioetanol dari alga hijau (chaetomorpha) dengan proses hidrolisa enzim dan fermentasi. Diploma thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Kebutuhan bahan bakar fosil dari setiap tahunnya selalu mengalami peningkatan, namun hal ini tidak diimbangi oleh bahan bakar fosil yang tersedia. Bioetanol (C2H5OH) salah satu bahan bakar alternativ dan terbarukan. Alga Hijau (Chaetomorpha) merupakan bahan baku yang dapat dijadikan bioetanol.
Tujuan dari penelitian ini adalah mengetahui potensi Alga Hijau (Chaetomorpha) untuk dijadikan etanol melalui proses hidrolisa enzim dan fermentasi.
Alga dikeringkan menggunakan oven pada suhu 60°C selama 24 jam, kemudian dihaluskan. Liquifikasi, tepung alga ditambahkan air hingga volume 2 liter, CaCl2 40 ppm, enzim α-amilase 2-5%(w/v) dan dipanaskan pada suhu 90°C-100°C selama 2 jam. Sakarifikasi, larutan ditambah HCl hingga pH 4, enzim glukoamilase 2-5% (w/v) dan dipanaskan pada suhu 60°C selama 4 jam. Fermentasi, larutan ditambahkan urea, yeast dan NPK dan diinkubasi selama 72 jam. Kemudian mendistilasi untuk mendapatkan etanolnya. Di setiap tahapnya dilakukan analisa kadar gula reduksi.
Hasil penelitian ini menunjukkan kadar gula reduksi setelah proses hidrolisa enzim pada konsentrasi alga 10% (w/v), 30% (w/v) dan 40% (w/v) dengan variasi penambahan enzim 2% dan 5% sebesar 19,64 g/l; 57,89 g/l; 64,62 g/l, 29,72 g/l; 91,67 g/l;dan 122,22 g/l. Konsentrasi alga optimum untuk proses fermentasi 72 jam diperoleh pada konsentrasi 40%(w/v) yang menghasilkan kadar etanol sebesar 8,16 %
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The demand of fossil fuel always increases every year, yet it is not offset by the available fossil fuel source. Bioethanol (C2H5OH) is one of the alternative and renewable fuels. Green algae (Chaetomorpha) is a potential raw material in bioethanol manufacture.
The aim of this study is to determine the potential of Green Algae (Chaetomorpha) to utilize as raw material in ethanol manufacture using enzymatic hydrolysis and fermentation process.
Alga is dried using oven at 60°C for 24 hours, then ground. Liquefaction, the algae flour is mixed with water until the solution volume is 2 litre, also 40 ppm CaCl2, 2-5%(w/v) α-amylase enzyme, and heatened at 90°C-100°C for 2 hours. Saccharification, HCl is added in the solution to increase the pH value to 4, 2-5% (w/v) glucoamylase enzyme, and heatened again at 60°C for 4 hours. Fermentation, the solution is mixed with urea, yeast, and NPK, and incubated for 72 hours. Then, the fermentation broth is distilled to obtain its ethanol content. The analysis of reducing sugar is conducted in every step of experiment.
This experimental result shows the reducing sugar content after enzymatic hydrolysis at algae concentration 10% (w/v), 30% (w/v), and 40% (w/v) and with variation of 2% and 5% enzyme addition is 19,64 g/l; 57,89 g/l; 64,62 g/l, 29,72 g/l; 91,67 g/l; and 122,22 g/l. The optimum algae concentration for 72 hours fermentation process is obtained at concentration 40%(w/v), which producing 8,16 % ethanol content.
| Item Type: | Thesis (Diploma) |
|---|---|
| Additional Information: | RSK 662.669 2 Wid p |
| Uncontrolled Keywords: | Chaetomorpha; Bbioetanol; Fermentasi; Enzim; Alga hijau; Reduksi; Kadar gula |
| Subjects: | T Technology > TP Chemical technology > TP156 Crystallization. Extraction (Chemistry). Fermentation. Distillation. Emulsions. |
| Divisions: | Faculty of Industrial Technology > Chemical Engineering > 24401-(D3) Diploma 3 |
| Depositing User: | - Taufiq Rahmanu |
| Date Deposited: | 01 Jul 2019 07:49 |
| Last Modified: | 01 Jul 2019 07:49 |
| URI: | http://repository.its.ac.id/id/eprint/63376 |
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