Halimah, Salma (2019) Pengaruh Penambahan Komposisi rGO Terhadap Biokompatibilitas dan Degradasi Hidrolitik Injectable Komposit Hidrogel Alginat/PVA/rGO untuk Aplikasi Tissue Engineering. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Rekayasa Jaringan berkembang dengan harapan mampu merekonstruksi fungsi dari jaringan yang rusak atau hilang, tanpa perlu merusak jaringan yang sehat.. Material yang digunakan untuk rekayasa jaringan adalah biomaterial. Biomaterial yang digunakan dalam penelitian ini adalah komposit alginat. Alginat merupakan kopolimer alami yang memiliki biokompatibilitas yang tinggi. Dalam penelitian ini akan dianalisa pengaruh penambahan Reduced Graphene Oxide (rGO) terhadap biokompatibilitas dan degradasi hidrolitik dari injectable komposit hidrogel Alginat/PVA/rGO. Sintesis komposit hidrogel alginat/PVA/rGO dimulai dengan sintesis rGO menggunakan Hummer’s Method, kemudian sintesis hidrogel dengan menggunakan CaSO4 sebagai crosslinker. Adapun variabel rGO yang diberikan pada hidrogel sebesar 0; 0,4; 0,8; 1,2; dan 1,6 dari total berat komposit alginat dan PVA. Pengujian SEM-EDX dilakukan untuk menganalisa struktur dan morfologi, untuk pengujian biokompatibilitas menggunakan MTT assay, dan pengujian anti bakteri. Analisa degradasi hidrolitik menggunakan pengujian weight loss. Dari pengujian diatas, jumlah penambahan rGO mempengaruhi morfologi hidrogel dengan membentuk struktur yang lebih rapat, serta didapatkan bahwa penambahan rGO sebesar 0,4 dari total berat komposit dapat meningkatkan nilai biokompatibilitas dan nilai viabilitas sel akan berkurang seiring dengan penambahan berat rGO, serta penambahan rGO belum memperlihatkan adanya zona inhibisi pada sekitar komposit. Nilai rasio degradasi hidrolitik mengalami penurunan setelah penambahan rGO. Modifikasi komposit alginat ini diharapkan mampu menjadi pendukung riset rekayasa jaringan, dikarenakan alginat serta PVA yang memiliki biokompatibilitas yang baik, serta penambahan rGO mampu mengoptimasi degradasi hidrolitik komposit hidrogel ini.
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Tissue engineering develops in the hope of being able to reconstruct the function of damaged or lost tissue, without the need to damage healthy tissue. The material used for tissue engineering is biomaterials. Biomaterials themselves act as a place for cell growth and development that has been adjusted to the design of the target tissue to be reconstructed. This is a reference for research on tissue engineering in the material world. The material used in this study was alginate composite. Alginate is a natural copolymer that has high biocompatibility. In this study we will analyze the effect of adding Reduced Graphene Oxide (rGO) to the biocompatibility and hydrolytic degradation of injectable composite hydrogel alginate / PVA / rGO. The synthesis of the hydrogel alginate / PVA / rGO composite was started by the synthesis of rGO using Hummer's Method, then the synthesis of the hydrogel using CaSO4 as a crosslinker. The rGO variable is 0; 0.4; 0.8; 1,2; and 1.6 of the composites total weight. SEM-EDX tests were carried out to analyze the structure and morphology, and for biocompatibility testing using MTT assay, and anti-bacterial testing. And the writer used weight loss testing to analyse hydrolytic degradation. From the above test, the number of additional rGO affects the morphology of the hydrogel by forming a more dense structure, and also the addition of 0,4 % rGO from the total composite weight can increase the cell viability value, but cell viability value decreases with the addition of rGO weight. In the degradation testing, the addition of rGO shows the zone of inhibition around the composite. The hydrolytic degradation ratio has decreased after the addition of rGO. The alginate composite modification is expected to be able for supporting tissue engineering research, because alginate and PVA have good biocompatibility, as well as the addition of rGO can optimize the hydrolytic degradation of these hydrogel composites.
| Item Type: | Thesis (Other) |
|---|---|
| Additional Information: | RSMt 620.2 Hal p-1 2019 |
| Uncontrolled Keywords: | Injectable, Hidrogel, Tissue Engineering, Alginat, PVA, rGO. |
| Subjects: | R Medicine > R Medicine (General) R Medicine > RD Surgery > RD130 Artificial organs; Prosthesis |
| Divisions: | Faculty of Industrial Technology > Material & Metallurgical Engineering > 28201-(S1) Undergraduate Thesis |
| Depositing User: | Salma Halimah |
| Date Deposited: | 06 Jun 2023 06:33 |
| Last Modified: | 06 Jun 2023 06:33 |
| URI: | http://repository.its.ac.id/id/eprint/65728 |
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