Aktivitas Antimikroba Ekstrak Daun Moringa oleifera

A`yunin, A`in Qurrota (2019) Aktivitas Antimikroba Ekstrak Daun Moringa oleifera. Masters thesis, Institut Teknologi Sepuluh Nopember.

Text 01211650010013-Master_Thesis.pdf Restricted to Repository staff only Download (1MB) | Request a copy

Abstract

Daun Moringa oleifera telah dimanfaatkan oleh masyarakat Indonesia sebagai obat tradisional. Daun M. oleifera mampu mengobati alergi atau infeksi oleh mikroba. Senyawa β-sitosterol telah berhasil diisolasi dari ekstrak metanol daun M. oleifera. Penentuan struktur senyawa ini didasarkan pada analisis data spektroskopi 1H-NMR, 13C-NMR, dan HMBC. Uji aktivitas antimikroba ekstrak n-heksana, diklorometana, etil asetat, dan metanol; serta fraksi n-heksana, etil asetat dan air dari ekstrak metanol daun M. oleifera menunjukkan bahwa ekstrak diklorometana dan fraksi etil asetat memiliki aktivitas antimikroba yang paling baik, yaitu sebesar 56,58% dan 53,45%; namun persentase penghambatannya rendah jika dibandingkan dengan kloramfenikol sebagai kontrol positif dengan penghambatan sebesar 100%. Uji aktivitas antimikroba β-sitosterol menunjukkan bahwa senyawa tersebut mampu menghambat pertumbuhan bakteri E.coli, R. palustris, S. epidermidis, P. aeruginosa, dan C. acnes dengan penghambatan berturut-turut sebesar 20,14%; 95%; 70,63%; 35,61%; dan 78,51%.
================================================================================================================================
Moringa oleifera leaves have been used by people in Indonesia as an traditional medicine. M. oleifera leaves is able to treat allergies or infections by microbes. β-Sitosterol compound was isolated from methanol extract of M. oleifera leaves. Structure elucidation of this compound was analyzed by 1H-NMR and 13C-NMR spectroscopy, and also HMBC. Antimicrobial activity assay of n-hexane, dichloromethane, ethyl acetate, and methanol exctracts; along with n-hexane, ethyl acetate and water fractions of methanol extract of M. oleifera leaves showed that dicloromethane extract and ethyl acetate fraction have the best antimicrobial activity, that is equal to 56,58% and 53,45%; but its have lower inhibition percentage compared with chloramphenicol as positive control with 100% inhibition. Antimicrobial activity of β-sitosterol showed that its compound was able to inhibit E.coli, R. palustris, S. epidermidis, P. aeruginosa, and C. acnes bacteria with 20,14%; 95%; 70,63%; 35,61%; and 78,51% inhibition, respectively.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	M. oleifera, isolasi, antimikroba, β-sitosterol
Subjects:	Q Science > QD Chemistry Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry Q Science > QD Chemistry > QD471 Chemical compounds - Structure and formulas
Divisions:	Faculty of Natural Science > Chemistry > 47101-(S2) Master Thesis
Depositing User:	A'in Qurrota A'yunin
Date Deposited:	20 Jul 2026 06:34
Last Modified:	20 Jul 2026 06:34
URI:	http://repository.its.ac.id/id/eprint/67699

Actions (login required)

View Item