Sari, Vivi Alvionita (2019) Degradasi Hidrogen Sulfida Dalam Limbah Pabrik Etanol oleh Bakteri Acidithiobacillus thiooxidans dan Pseudomonas putida dengan Metode Biofiltrasi Aerobik Dalam Bioreaktor. Masters thesis, Institut Teknologi Sepuluh Nopember.
![[thumbnail of 02211750010015-Master_Thesis.pdf]](http://repository.its.ac.id/style/images/fileicons/text.png) |
Text
02211750010015-Master_Thesis.pdf Restricted to Repository staff only Download (9MB) | Request a copy |
Abstract
Molase, Stillage dan Spent wash merupakan limbah cair dalam Industri bioethanol dimana limbah tersebut diproduksi dalam kuantitas yang besar. Ketiga bahan tersebut mengandung unsur sulfur yang tinggi dan berbahaya jika dibuang langsung ke lingkungan tanpa pengolahan terlebih dahulu. Penelitian ini bertujuan untuk menghilangkan kandungan H2S atau sulfur dalam limbah cair pabrik etanol dengan memanfaatkan mikroorganisme melalui biofiltrasi dengan menggunakan mikroorganisme Acidithiobacillus thiooxidans dan Pseudomonas putida yang diimobilisasi pada packing serpihan kayu dan ampas tebu. Proses biofiltrasi pada penelitian ini,ada 2 tahap. Tahap pertama dilakukan imobilisasi pada packing untuk membentuk biofilm selama 13 hari dan tahap kedua proses biofiltrasi pada limbah molase, stillage dan spent wash selama 30 hari. Dari hasil penelitian ini, didapatkan data bahwa penurunan kadar H2S yang terbaik adalah pada variabel penambahan 30% (v/v) Acidithiobacillus thiooxidans dalam packing kayu dalam limbah molase (konsentrasi 3% (v/v)) dapat menurunkan konsentrasi H2S dari 4.35 mg/L menjadi 0.91 mg/L dengan presentase degradasi sebesar 79.08%. Presentase degradasi dari limbah stillage dan spent wash lebih rendah dibandingkan dengan limbah molase. Hal ini dikarenakan stillage dan spent wash mengandung alkohol (stillage : 242.81 mg/L, spent wash : 263.85 mg/L) yang bersifat antiseptik bagi bakteri.
======================================================================================================================================
Molasses, Stillage and Spent wash are liquid wastes as large production in the bioethanol industry. The liquid wastes contain high sulfur and are dangerous if they are discharge directly into the environment without pretreatment. For this reason, pretreatment is needed to reduce the sulfur concentration. The objective of this research is to eliminate the content of H2S or sulfur in the wastewater of the bioethanol industry by using aerobic bacteria such as Acidithiobacillus thiooxidans and Pseudomonas putida which are immobilized packing wood chips and bagasse in biofiltration reactor to degraded H2S. The biofiltration process in this study conducted in two steps. The first step was carried out by immobilized bacterial in the reactor to form biofilm for 13 days and the second step of the biofiltration process used wastewater of the bioethanol industry (molasses, stillage, and spent wash) for 30 days. From this study, showed that the best result for degrading sulfur were 30% (v/v) bacteria Acidithiobacillus thiooxidans in wood chips packing and 3% (v/v) concentration molasses waste could reduce H2S concentration from 4.35 mg/L to 0.91 mg/L with 79.08% total degradation. The percentage of degradation from stillage and spent wash was lower compared to molasses. It is because the stillage and spent wash contain alcohol (stillage : 242.81 mg/L and spent wash : 263.85 mg/L) which is antiseptic for bacteria.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Acidithiobacillus thiooxidans, Biofiltration, Molasses, Pseudomonas putida, Stillage, Spent wash |
| Subjects: | Q Science Q Science > QR Microbiology |
| Divisions: | Faculty of Industrial Technology > Chemical Engineering > 24101-(S2) Master Thesis |
| Depositing User: | Sari Vivi Alvionita |
| Date Deposited: | 22 Jul 2026 02:18 |
| Last Modified: | 22 Jul 2026 02:18 |
| URI: | http://repository.its.ac.id/id/eprint/69411 |
Actions (login required)
 |
View Item |