Nury, Dennis Farina (2019) Pemanfaatan Ekstrak Daun Binahong (Anredera cordifolia (Tenore) Steenis Termodifikasi Polipirol Untuk Biosensor. Masters thesis, Institut Teknologi Sepuluh Nopember Surabaya.
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Abstract
Modifikasi elektroda emas menggunakan polipirol dan ekstrak daun binahong untuk mendeteksi glukosa telah berhasil dilakukan. Biosensor glukosa dibuat dengan cara melapiskan bahan aktif pada permukaan elektroda emas. Bahan aktif yang digunakan adalah polipirol (Ppy) dan ekstrak daun binahong. Polimerisasi pirol dilakukan secara elektrokimia pada rentang potensial -1,3 hingga 1,3 V menggunakan metode voltammetri siklik dengan laju sapuan 50 mV/s sebanyak 30 siklus dalam larutan elektrolit KCl 0,1 M. Elektroda emas termodifikasi selanjutnya diuji dengan membandingkannya dengan elektroda emas tanpa modifikasi, elektroda emas termodifikasi polipirol, dan elektroda emas termodifikasi polipirol serta ekstrak daun binahong untuk mendeteksi senyawa glukosa. Perbandingan dilakukan berdasarkan respons arus yang dihasilkan terhadap larutan analit dengan konsentrasi yang sama, yaitu 10 mM, pada variasi pH 4, 7, dan 10. Berdasarkan hasil pengukuran, elektroda yang dimodifikasi dengan polipirol dan ekstrak daun binahong mampu mendeteksi larutan glukosa dengan baik pada pH 7. Hal ini dibuktikan dengan terbentuknya puncak anodik dan katodik pada voltamogram yang dihasilkan pada potensial 0,16 V dengan puncak arus oksidasi sebesar 2,4 μA. Kurva kalibrasi hasil pengukuran variasi konsentrasi glukosa digunakan untuk menentukan limit deteksi dan sensitivitas elektroda terhadap larutan tersebut. Berdasarkan hasil perhitungan, limit deteksi dan sensitivitas elektroda emas termodifikasi polipirol dan ekstrak daun binahong terhadap glukosa yang diukur berdasarkan respons arus puncak oksidasi berturut-turut adalah 4,41 mM dan 0,315 μA mm⁻² mM⁻¹.
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Modification of gold electrodes using polypyrrole and binahong leaf extract for glucose detection was successfully carried out. The glucose biosensor was prepared by coating the active materials onto the surface of a gold electrode. The active materials used were polypyrrole (Ppy) and binahong leaf extract. Pyrrole polymerization was carried out electrochemically at a potential range of -1.3 to +1.3 V using the cyclic voltammetry method, with a scan rate of 50 mV/s for 30 cycles in a 0.1 M KCl electrolyte solution. The modified gold electrode was subsequently tested by comparing it with an unmodified gold electrode, a polypyrrole-modified gold electrode, and a gold electrode modified with polypyrrole and binahong leaf extract for glucose detection. The comparison was based on the current response produced by an analyte solution with the same concentration of 10 mM at varying pH values of 4, 7, and 10. Based on the measurement results, the electrode modified with polypyrrole and binahong leaf extract was able to detect glucose solution effectively at pH 7. This was demonstrated by the formation of anodic and cathodic peaks in the resulting voltammogram at a potential of 0.16 V, with an oxidation peak current of 2.4 μA. The calibration curve obtained from measurements at various glucose concentrations was used to determine the detection limit and sensitivity of the electrode toward the analyte solution. Based on the calculations, the detection limit and sensitivity of the gold electrode modified with polypyrrole and binahong leaf extract toward glucose, determined from the oxidation peak current response, were 4.41 mM and 0.315 μA mm⁻² mM⁻¹, respectively.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | binahong, biosensor, glukosa, polipirol, voltametri |
| Subjects: | Q Science Q Science > QD Chemistry Q Science > QD Chemistry > QD553 Electrochemistry. Electrolysis T Technology > TP Chemical technology > TP255 Electrochemistry, Industrial. |
| Divisions: | Faculty of Industrial Technology > Chemical Engineering > 24101-(S2) Master Thesis |
| Depositing User: | Nury Dennis Farina |
| Date Deposited: | 22 Jul 2026 01:29 |
| Last Modified: | 22 Jul 2026 01:29 |
| URI: | http://repository.its.ac.id/id/eprint/69464 |
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