Potensi azotobacter sebagai penghasil hormon pertumbuhan auksin

Kholida, Fahima (2015) Potensi azotobacter sebagai penghasil hormon pertumbuhan auksin. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Azotobacter memiliki kelebihan dibandingkan dengan bakteri rhizosfer lainnya karena Azotobacter dapat menambat N atmosferik. Azotobacter juga mampu mensintesis hormon pertumbuhan IAA, golongan auksin alami yang paling melimpah dan terlibat dalam regulasi pertumbuhan dan perkembangan tanaman. Berdasarkan kemampuannya tersebut dilakukan penelitian untuk menguji potensi Azotobacter yang diisolasi dari Eco Urban Farming ITS sebagai penghasil IAA. Uji kualitatif dilakukan untuk penapisan Azotobacter yang mempunyai indikasi menghasilkan hormon IAA dengan metode Salkowksi pada media NA triptofan. Selanjutnya dilakukan uji kuantitatif untuk mengetahui konsentrasi hormon IAA yang mampu dihasilkan oleh isolat Azotobacter dengan metode determinasi kolorimetri yang ditunjukkan dengan pereaksi Salkowksi pada media Nutrient Broth yang disuplementasi triptofan. IAA yang terbentuk diukur menggunakan spektrofotometer dengan λ=530 nm. Isolat yang mampu menghasilkan IAA adalah Azotobacter A1a, A1b, A3, A6, A9, dan A10. Isolat Azotobacter A1a, A9 dan A10 dapat memproduksi IAA lebih besar dibanding yang lain. Konsentrasi IAA terbesar dihasilkan oleh isolat Azotobacter A10 mencapai 9,45 ppm, diikuti isolat Azotobacter A9 sebesar 7,38 ppm dan isolat Azotobacter A1a sebesar 5,40 ppm. ================================================================================================ Azotobacter has a beneficial value compared with other rhizosphere bacteria because Azotobacter can fix atmospheric nitrogen. Azotobacter is also able to synthesize growth hormone IAA, the most abundant natural auxin group,and it is involved in the regulation of plant growth and development. Based on that potential, the research was conducted to test the potential Azotobacter which was isolated from Eco Urban Farming ITS, as a producer of IAA. Qualitative test was done to screen Azotobacter that indicate producing hormones IAA with Salkowksi method on NA tryptophan. Then, quantitative test was done to measure the amount of the concentration of IAA hormone which was produced by Azotobacter isolate using colorimetric determination method, which was shown by Salkowksi reagent on Nutrient Broth medium, that is supplemented by tryptophan. Formed IAA was measured using spectrophotometer λ=530nm. The result shown that the isolate that can produce IAA is Azotobacter A1a, A1b, A3, A6, A9, and A10. Azotobacter A1a, A9 and A10 can produce IAA higher than others. The largest concentration of IAA produced by isolate Azotobacter A10 which can reach 9.45 ppm, followed by A9 isolate Azotobacter with 7.38 ppm and isolate Azotobacter A1a with 5.40 ppm.

Item Type: Thesis (Undergraduate)
Additional Information: RSBi 571.849 29 Kho p
Uncontrolled Keywords: Azotobacter, hormon auksin, IAA, konsentrasi
Subjects: Q Science > QH Biology > QH301 Biology
Divisions: Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User: - Taufiq Rahmanu
Date Deposited: 18 Nov 2019 07:48
Last Modified: 18 Nov 2019 07:48
URI: http://repository.its.ac.id/id/eprint/71855

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