Uji efektivitas ekstrak buah dan daun cabe jawa (piper retrofractum vahl) terhadap larva nyamuk culex sp. sebagai larvasida

Hotimah, Hosnul (2015) Uji efektivitas ekstrak buah dan daun cabe jawa (piper retrofractum vahl) terhadap larva nyamuk culex sp. sebagai larvasida. Undergraduate thesis, Institut Teknologi Sepuluh Nopember Surabaya.

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Abstract

Piper retrofractum Vahl. atau cabe jawa merupakan tanaman asli Indonesia. Berdasarkan penelitian yang telah dilakukan oleh Chansang et al., (2005), menunjukkan bahwa ekstrak buah cabe jawa sangat berpotensi untuk mengontrol larva nyamuk, karena kandungan bioaktif dan biofungsionalnya seperti dibutilamida dan pipericide. Penelitian ini bertujuan untuk mengetahui bagaimana keefektifan dari dua jenis ekstrak larvasida yaitu dari daun dan buah cabe jawa terhadap larva nyamuk Culex sp..
Proses ekstraksi pada penelitian ini menggunakan metode maserasi dengan pelarut etanol 95%. Keefektifan ekstrak ditinjau dari nilai LC50 pada data persentase (%) kematian larva dan persentase (%) pembentukan pupa. Kemudian data yang diperoleh dianalisis Probit untuk mengetahui potensi larvasida dari kedua ekstrak terhadap larva Culex sp. dan dinyatakan sebagai Lethal Concentration (LC), ANOVA One Way untuk mengetahui pengaruh konsentrasi ekstrak terhadap jumlah kematian larva dan uji Tukey untuk mencari perbedaan secara signifikan antara konsentrasi tersebut dengan taraf kepercayaan 95%.
Hasil penelitian menujukkan bahwa ekstrak buah cabe jawa jauh lebih efektif dibandingkan ekstrak daun cabe jawa dalam menghambat larva nyamuk Culex sp.. Berdasarkan nilai LC50 yang telah diketahui, ekstrak buah cabe jawa memiliki nilai LC50 sebesar 6,96 ppm dan nilai LC50 ekstrak daun cabe jawa, yaitu 3071,67 ppm. Menurut WHO (2009), suatu senyawa dikatakan aktif pada uji larvasida jika nilai LC50 yang diperoleh <100 ppm.

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Piper retrofractum Vahl. or Javanese long pepper is native plant in Indonesia. Based on research that conducted by Chansang et al., (2005), showed that fruit extract of Javanese long pepper has the potential to control mosquito larvae, because have the content of bioactive and biofunctional like dibutilamide and pipericide. The purpose of this study is to determine how the effectiveness of two types of larvicide extracts from the leaves and fruit of Javanese long pepper, against mosquito Culex sp. larvae.
The extraction process in this study using maceration method with ethanol 95%. And then the effectiveness of extract observed by LC50 values from the percentage (%) data of death and the percentage (%) pupa formation. Then the data were Probit analyzed to know the potential of larvicide from this extract against Culex sp. larvae and expressed as Lethal Concentration (LC), ANOVA One Way to know the effect of the concentration extract to the number of larvae mortality and the Tukey test to find the significant differences between those with a concentration level of 95%.
The results showed that the fruit extract of Javanese long pepper was more effective than Javanese long pepper leaf extract in inhibiting larvae of the mosquito Culex sp.. The value of LC50 Javanese long pepper fruit extracts was 6.96 ppm and the value of LC50 extract Javanese long pepper leaves was 3071.67 ppm. According to WHO (2009), a compound to be an active in the larvicide test if the value obtained LC50 <100 ppm.

Item Type: Thesis (Undergraduate)
Additional Information: RSBi 576 Hot u
Uncontrolled Keywords: cabe jawa, ekstrak., LC50, mortalitas, Culex sp.
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User: - Taufiq Rahmanu
Date Deposited: 20 Nov 2019 07:22
Last Modified: 20 Nov 2019 07:22
URI: http://repository.its.ac.id/id/eprint/71918

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