Sriningsih, Atik (2015) Potensi isolat bakteri Pseudomonas dan yeast sebagai pendegrasi plastik. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Plastik merupakan salah satu polimer sintetik atau
buatan manusia yang merupakan rantai panjang molekul
polimer. Polietilen adalah polimer sintetik yang memiliki tingkat
hidrofobik dan berat molekul yang tinggi. Polimer ini sering
digunakan sebagai kantong plastik, pengemasan barang, susu
dan botol air minum yang menyebabkan masalah lingkungan.
Biodegradasi adalah proses dimana mikroorganisme mampu
mendegradasi atau memecah polimer alam (seperti lignin dan
selulosa) dan polimer sintetik (seperti polietilen dan polistiren).
Pseudomonas merupakan genus mikroorganisme yang
dapat ditemukan dimanapun dan hidup bebas, hal ini berkaitan
dengan kebutuhan nutrisi dan kisaran senyawa karbon yang
digunakannya cukup sederhana, serta kemampuan adaptasi
genetik dan metabolik yang cukup tinggi. Pseudomonas secara
umum tidak memiliki enzim hidrolitik yang penting dalam
mendegradasi polimer menjadi monomer namun bakteri ini
memiliki system inducible operon yang mampu menghasilkan
enzim tertentu dalam proses metabolisme sumber karbon yang
tidak biasa digunakan.
Yeast merupakan jamur uniseluler. Salah satu karakter
yang umum diketahui adalah kemampuan untuk memfermentasi
gula menjadi etanol. Genus Pseudozyma dan Cryptococcus
merupakan contoh genus yeast yang biasa dimanfaatkan dalam
proses degradasi plastik biodegradable. Penelitian ini bertujuan untuk mengetahui kemampuan
bakteri Pseudomonas hasil inkubasi dari penelitian sebelumnya,
isolat Pseudomonas L1 dan Yeast Candida M2.3 dan Yeast
Debaryomyces R1.10 murni dalam proses degradasi plastik
tanpa membedakan jenis plastik uji.
Inokulum uji diinkubasi dalam botol yang berisi Mineral
Salt Medium (MSM), pasir steril dan plastik uji. Inkubasi
dilakukan selama 3 bulan. Parameter yang diamati adalah
persentase kehilangan berat kering, Optical Density dan pH yang
diukur setiap 3 minggu sekali secara destruktif. Data yang
didapat dianalisis menggunakan ANOVA One Way dengan
tingkat kepercayaan 0.05.
Hasil ANOVA One Way menunjukkan bahwa jenis
inokulum tidak berpengaruh secara nyata terhadap proses
degradasi plastik. Berdasarka persentase kehilangan berat
keringnya konsorsium PY1( Pseudomonas L1 dan Yeast M2.3
Candida) memberikan nilai persentase kehilangan berat kering
paling besar pada plastik putih sebesar 5,6-7,6%.
========================================================================================================
Plastic is one of synthetic polymer or man-made that is a
long chain of polymer molecules. Polyethylene is synthetic
polymer that has the hydrophobic and high molecular weight .This
polymer often used as a plastic shopping bag , packaging of goods ,
milk and a bottle of drinking water that causes environmental
problems .Biodegradation is the process whereby microorganisms
capable to degrades or break up a polymer nature (like lignin and
cellulose) and synthetic polymer (as polyethylene and
polystyrene).
Pseudomonas is a genus of truly ubiquitous organisms,
which seems to be a consequence of their simple nutritional
requirements, the range of carbon compounds they utilize, and their
genetic and metabolic adaptability. Pseudomonas generally not
having hydrolytic action of enzymes important degrades
monomers in a polymer but this bacteria have systems called
inducible operon that has capability to producing certain enzymes
in metabolic processes of carbon unusual used.
Yeast is unicellular fungi .One of the common character
known is the ability to ferment the sugar to ethanol. Cryptococcus
and Pseudozyma are genus of the yeast that are commonly used in
the degradation of biodegradable plastic process.
This research was conducted to find out the capabilities of
Pseudomonas Kx1 incubation results from previous studies,
isolates of Pseudomonas L1 and pure yeast in the plastic
degradation process. Inoculumes are incubated in a bottle test that contains
mineral salt medium (MSM), sand sterile and plastics test.
Incubation done for 3 months. The parameters that observed are the
percentages of dry lose weight , optical density and pH which is
measured every three weeks in destructive. Data obtained analyzed
using ANOVA one-way with the p value 0.05 .
The research aims to understand the ability of any kind of
inoculume plastic degrades in without differentiating the plastic
type. The results of one-way ANOVA shows that kind of inokulum
not influence significantly to the degradation of plastic. Based on
the percentage lost the weight of drying up of the consortium PY1
(Pseudomonas and yeast Candida M2.3) give the value of the
percentage lost dry weight of white plastic by 5,6 -7,6 percent .
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSBi 579.3 Sri p |
| Uncontrolled Keywords: | biodegradasi, plastik, Pseudomonas, yeast |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | - Taufiq Rahmanu |
| Date Deposited: | 21 Nov 2019 07:07 |
| Last Modified: | 21 Nov 2019 07:07 |
| URI: | http://repository.its.ac.id/id/eprint/71948 |
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