Pengaruh asam salisilat dan fenilalanin terhadap kandungan total asam fenol pada kultur suspensi sel moringa oleifera lam

Permanasari, Yunita (2015) Pengaruh asam salisilat dan fenilalanin terhadap kandungan total asam fenol pada kultur suspensi sel moringa oleifera lam. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Moringa oleifera merupakan tanaman yang berpotensi tinggi untuk dikembangkan dalam sektor industri seperti industri farmasi karena kandungan senyawa fenolnya yang tinggi. Tujuan dari penelitian ini untuk mengetahui pengaruh asam salisilat sebagai elisitor dan phenilalanin sebagai prekursor terhadap kandungan total asam fenol pada kultur suspensi sel M. oleifera. Rancangan penelitian yang digunakan adalah RAL (rancangan acak lengkap) dengan ulangan sebanyak 3 kali. Parameter uji yang dilakukan adalah estimasi senyawa total asam fenol menggunakan spektrofotometer UV-Vis dan berat kering hasil perlakuan asam salisilat dan fenilalanin. Hasil dianalisa menggunakan anova one way dan uji Tukey. Hasil penelitian menunjukkan variasi konsentrasi asam salisilat dan fenilalanin berpengaruh terhadap peningkatan kandungan total asam fenol pada kultur suspensi sel M. oleifera Total asam fenol tertinggi terdapat pada asam salisilat 1 ppm 1,6067± 0,458 ppm dan fenilalanin 5 ppm 1,60 ± 0,37 ppm. ============================================================================================================== Moringa oleifera is a plant that has high potential to be developed in industrial sectors such as the pharmaceutical industry because of the high content of phenol compounds. The purpose of this study was to determine the effect of salicylic acid as elicitor and phenylalanine as a precursor to the total content of phenolic acids in cell suspension cultures of M. oleifera. The research design used was RAL (completely randomized design) with 3 replications. Test parameters the estimation of the total phenolic acids compounds using UVVis spectrophotometer and the dry weight of salicylic acid and phenylalanine treatment. The result were analyzed by oneway ANOVA and Tukey test. The results showed that varying concentration of salicylic acid and phenylalanine affect the increase of the total content of phenolic acids in cell suspension cultures of M. oleifera. The highest total phenolic acids contained at concentration of salicylic acid 1 ppm was 1.6067 ± 0.458 ppm and phenylalanine 5 ppm was 1,60 ± 0.37 ppm

Item Type: Thesis (Undergraduate)
Additional Information: RSBi 583 64 Per p
Uncontrolled Keywords: asam fenol ,asam salisilat, fenilalanin, M. oleifera
Subjects: Q Science > QH Biology > QH75 Nature conservation. Landscape protection. Biodiversity conservation. Endangered species and ecosystems (General). Habitat conservation. Ecosystem management. Conservation biology
Divisions: Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User: - Taufiq Rahmanu
Date Deposited: 21 Nov 2019 07:15
Last Modified: 21 Nov 2019 07:15
URI: http://repository.its.ac.id/id/eprint/71950

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