Rohmah, Eka Afiyanti (2016) Analisis Pertumbuhan Tanaman Kedelai (Glycine Max L.) Varietas Grobogan Pada Perlakuan Cekaman Genangan. Undergraduate thesis, Institut Teknologi Sepuluh Nopember Surabaya.
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Abstract
Tanaman Kedelai (Glycine max L.) merupakan tanaman
pangan yang penting terkait kandungan nutrisinya, terutama
kandungan protein yang tinggi. Kebutuhan yang meningkat tidak
diimbangi dengan peningkatan produksinya. Salah satunya
disebabkan oleh cekaman genangan.
Penelitian ini dilakukan dengan pemaparan kedelai
selama 14 hari dalam media kontrol (100%), dan media yang di
beri perlakuan genangan dengan konsentrasi genangan 125%,
150%, 175% dan 200%. Parameter yang diukur meliputi tinggi
tanaman, jumlah cabang, luas daun, berat basah dan berat
kering, panjang akar tanaman, jumlah akar adventif, serta profil
protein. Analisa profil protein dilakukan dengan metode
elektroforesis SDS-PAGE.
Hasil yang didapat menunjukkan bahwa terjadi
penurunan pada beberapa parameter pertumbuhan. Penurunan
paling signifikan terjadi pada perlakuan cekaman genangan
dengan konsentrasi 200%. Secara berturut-turut, untuk
parameter luas daun, berat basah dan berat kering serta panjang
akar tanaman sebesar 15.99 cm2, 3.16 g, 0.59 g, 15.38 cm.
Parameter akar adventif mengalami peningkatan seiring dengan
peningkatan konsentrasi cekaman. Peningkatan jumlah akar
adventif tertinggi terjadi pada genangan 200% dengan nilai
tertinggi 18,00. Hasil analisis profil protein menunjukkan
terdapat pita protein baru dengan berat molekul 39,94 kDa;
44,63; 59,38; 66,04 kDa. Keempat protein tersebut diduga
merupakan protein yang terekspresi akibat adanya induksi
cekaman genangan.
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Soybean Plants (Glycine max L.) is an important food
crop related to its nutritional content, especially high protein
content. Although increment of need is not matched by an
increase in production. One of them caused by waterlogging
stress.
This research was conducted with soy exposure for 14
days in the media controls (100%), and the media were given
treatment waterlogging stress with a concentration of 125%,
150%, 175% and 200%. Parameters measured were plant height,
number of branches, leaf area, fresh weight and dry weight, root
length, number of adventitious roots, and protein profiles.
Analysis of protein profiles by SDS-PAGE electrophoresis
method.
The results showed that a decline in all parameters of
growth. The most significant decrease occurred in the treatment
of waterlogging stress with a concentration of 200%.
Respectively, for the parameters leaf area, fresh weight and dry
weight and length of plant roots by 15.997 cm2, 3.168 g, 0.595 g,
15.383 cm. Parameter adventitious roots increased in line with
increased concentrations of stress. Increasing the number of
adventitious roots was highest of 200% with the highest value of
18. The results of analysis of protein profiles showed that new
protein band with a molecular weight of 39.94 kDa; 44.63 kDa;
59,38 kDa; 66.04 kDa. All those fourth expressed protein is
thought to be due to stress induced waterlogging.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSBi 571.849 29 Roh a |
| Uncontrolled Keywords: | Cekaman Genangan, Glycine max L, SDS-PAGE |
| Subjects: | Q Science > QH Biology > QH426 Genetics |
| Divisions: | Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | EKO BUDI RAHARJO |
| Date Deposited: | 26 Nov 2019 06:11 |
| Last Modified: | 26 Nov 2019 06:11 |
| URI: | http://repository.its.ac.id/id/eprint/72038 |
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