Analisis Pertumbuhan Tanaman Kedelai (Glycine Max L.) Varietas Grobogan Pada Perlakuan Cekaman Genangan

Rohmah, Eka Afiyanti (2016) Analisis Pertumbuhan Tanaman Kedelai (Glycine Max L.) Varietas Grobogan Pada Perlakuan Cekaman Genangan. Undergraduate thesis, Institut Teknologi Sepuluh Nopember Surabaya.

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Abstract

Tanaman Kedelai (Glycine max L.) merupakan tanaman pangan yang penting terkait kandungan nutrisinya, terutama kandungan protein yang tinggi. Kebutuhan yang meningkat tidak diimbangi dengan peningkatan produksinya. Salah satunya disebabkan oleh cekaman genangan. Penelitian ini dilakukan dengan pemaparan kedelai selama 14 hari dalam media kontrol (100%), dan media yang di beri perlakuan genangan dengan konsentrasi genangan 125%, 150%, 175% dan 200%. Parameter yang diukur meliputi tinggi tanaman, jumlah cabang, luas daun, berat basah dan berat kering, panjang akar tanaman, jumlah akar adventif, serta profil protein. Analisa profil protein dilakukan dengan metode elektroforesis SDS-PAGE. Hasil yang didapat menunjukkan bahwa terjadi penurunan pada beberapa parameter pertumbuhan. Penurunan paling signifikan terjadi pada perlakuan cekaman genangan dengan konsentrasi 200%. Secara berturut-turut, untuk parameter luas daun, berat basah dan berat kering serta panjang akar tanaman sebesar 15.99 cm2, 3.16 g, 0.59 g, 15.38 cm. Parameter akar adventif mengalami peningkatan seiring dengan peningkatan konsentrasi cekaman. Peningkatan jumlah akar adventif tertinggi terjadi pada genangan 200% dengan nilai tertinggi 18,00. Hasil analisis profil protein menunjukkan terdapat pita protein baru dengan berat molekul 39,94 kDa; 44,63; 59,38; 66,04 kDa. Keempat protein tersebut diduga merupakan protein yang terekspresi akibat adanya induksi cekaman genangan. =========================================================== Soybean Plants (Glycine max L.) is an important food crop related to its nutritional content, especially high protein content. Although increment of need is not matched by an increase in production. One of them caused by waterlogging stress. This research was conducted with soy exposure for 14 days in the media controls (100%), and the media were given treatment waterlogging stress with a concentration of 125%, 150%, 175% and 200%. Parameters measured were plant height, number of branches, leaf area, fresh weight and dry weight, root length, number of adventitious roots, and protein profiles. Analysis of protein profiles by SDS-PAGE electrophoresis method. The results showed that a decline in all parameters of growth. The most significant decrease occurred in the treatment of waterlogging stress with a concentration of 200%. Respectively, for the parameters leaf area, fresh weight and dry weight and length of plant roots by 15.997 cm2, 3.168 g, 0.595 g, 15.383 cm. Parameter adventitious roots increased in line with increased concentrations of stress. Increasing the number of adventitious roots was highest of 200% with the highest value of 18. The results of analysis of protein profiles showed that new protein band with a molecular weight of 39.94 kDa; 44.63 kDa; 59,38 kDa; 66.04 kDa. All those fourth expressed protein is thought to be due to stress induced waterlogging.

Item Type: Thesis (Undergraduate)
Additional Information: RSBi 571.849 29 Roh a
Uncontrolled Keywords: Cekaman Genangan, Glycine max L, SDS-PAGE
Subjects: Q Science > QH Biology > QH426 Genetics
Divisions: Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User: EKO BUDI RAHARJO
Date Deposited: 26 Nov 2019 06:11
Last Modified: 26 Nov 2019 06:11
URI: http://repository.its.ac.id/id/eprint/72038

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