Hidrolisis Trigliserida Pada Fraksi Non Polar Dari Minyak Nyamplung Menggunakan Rhizopus Oryzae Dan Lactobacillus Plantarum - Triacylglycerols Hydrolysis From Non Polar Fraction Of Nyamplung (Calophyllum Inophyllum) Oil Using Rhizopus Oryzae And Lactobacillus Plantarum

Tahfifah, Amirut and Lestari, Hilda Dwi (2016) Hidrolisis Trigliserida Pada Fraksi Non Polar Dari Minyak Nyamplung Menggunakan Rhizopus Oryzae Dan Lactobacillus Plantarum - Triacylglycerols Hydrolysis From Non Polar Fraction Of Nyamplung (Calophyllum Inophyllum) Oil Using Rhizopus Oryzae And Lactobacillus Plantarum. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Minyak dari biji Calophyllum inophyllum (nyamplung) umumnya hanya digunakan sebagai bahan baku biodiesel, padahal terdapat banyak manfaat yang lain. Fraksi lipid non polar pada minyak Calophyllum inophyllum mengandung trigliserida tinggi dan beberapa komponen bioaktif. Trigliserida dapat dihidrolisis untuk mendapatkan asam-asam lemak penyusunnya dan gliserol. Penelitian ini bertujuan untuk mengetahui pengaruh jenis mikroorganisme penghasil enzim (whole-cell) pada reaksi hidrolisis trigliserida, mengetahui pengaruh jumlah mikorganisme penghasil enzim (whole-cell) pada reaksi hidrolisis trigliserida, dan mengetahui pengaruh waktu pada reaksi hidrolisis trigliserida. Minyak nyamplung diekstraksi multi stage sebanyak 8 kali, rasio pelarut terhadap minyak 5:1 (g/g), dan rasio pelarut Petroleum eter-methanol 3:1 (g/g). NPLF yang didapat kemudian dianalisa kandungan trigliserida,digliserida, monogliserida dan FFA. Mikroorganisme yang digunakan adalah R.oryzae tanpa melalui rekayasa genetika ditanam dan Lactobacillus plantarum. Proses hidrolisa dilakukan dengan menambahkan air dengan perbandingan NPLF:air sebesar 1:3(g/g) dan mikoorganisme sesuai variable. Variabel R.oryzae yang diberikan adalah 1012 sel/mL mixture, 5x1012 sel/mL mixture, dan 10x1012sel sel/mL mixture. Campuran tersebut dimasukkan pada inkubator shaker pada suhu 300C, kecepatan 150 rpm selama 1 hari, 2 hari, 3 hari, 4 hari, dan 5 hari. NPLF dan fraksi minyak hasil hidrolisis akan dianalisa secara kualitatif menggunakan TLC dan spektrofotometri UV-Vis. Kadar trigliserida, digliserida, dan monogliserida dianalisa menggunakan metode kolom kromatografi. Untuk analisa trigliserida, setelah metode kolom kromatografi dilanjutkan dengan titrasi oksidialkalimetri lalu menggunakan spektrofotometri panjang gelombang 285 mμ. Sedangkan kadar FFA dianalisa secara kuantitaif menggunakan metode titrasi asidi-alkalimetri. Hasil percobaan ekstraksi likuid-likuid minyak nyamplung memiliki kandungan sebagai trigliserida sebesar 94%, digliserida sebesar 2,01%, monogliserida sebesar 0,37%, dan FFA sebesar 3,43%. Dari hasil hidrolisa dapat diketahhui bahwa pada saat kadar FFA (free fatty acid) < 1% dan kadar digliserida <0,45% akan terjadi proses hidrolisa. Kesimpulan dari penelitian yang telah dilakukan antara lainb: penggunaan R.Oryzae lebih efektif dibandingkan L.Plantarum untuk proses hidrolisa trigliserida dari NPLF minyak nyamplung. Jumlah whole-cell microbial tidak berpengaruh pada proses. Lama waktu hidrolisa akan mempengaruhi kadar FFA (free fatty acid) yang terbentuk karena semakin lama proses hidrolisa dapat menyebabkan adanya reaksi balik ataupun reaksi esterifikasi, sehingga kadar trigliserida akan naik dan kadar FFA justru turun. ======================================================================================================================== Oil from the seeds of Calophyllum inophyllum (nyamplung) is generally only used as raw material for biodiesel, but there are many other benefits beside it. Non polar lipid fraction in Calophyllum inophyllum oil contains high triglycerides and several bioactive components. Triglycerides can be hydrolyzed to obtain constituent fatty acids and glycerol. This study aims to determine the effect of microorganisms type for producing enzymes using whole-cell biocatalyst on triglyceride hydrolysis reaction, knowing the effect of the number of mikorganisme producing enzymes whole-cell biocatalyst on the hydrolysis of triglycerides, and determine the effect of time on the hydrolysis of triglycerides. Purification of Nyamplung oil is using multi stage oil extracted 8 times, solvent to oil ratio is 5: 1 (g / g), and the ratio of solvent Petroleum ether-methanol is 3: 1 (g / g). Non polar lipid fraction were obtained and then analyzed the content of triglycerides, diglycerides, monoglycerides and FFA. The microorganism used are Rhizopus oryzae grown without genetic engineering and Lactobacillus plantarum. Hydrolysis process is done by adding water at a ratio of NPLF:water is 1: 3 (g / g) and microorganisms corresponding variable. Number of R.oryzae and L.plantarum given are 1012 cells/mL mixture, 5x1012 cells/mL mixture, and 10x1012 cells/mL mixture. The mixture was put in an incubator shaker at a temperature of 300C, speed 150 rpm for 1 day, 2 days, 3 days, 4 days, and 5 days. NPLF and oil fractions hydrolysis results will be analyzed qualitatively using TLC and UV-Vis spectrophotometry. High levels of triglycerides, diglycerides, monoglycerides and analyzed using column chromatography. For analysis of triglycerides, after the method of column chromatography followed by oksidialkalimetri titration and use spectrophotometric wavelength of 285 mμ. While the quantitative levels of FFA were analyzed using acidbase titratio. The experimental results of liquid-liquid extraction nyamplung oil contains as triglycerides 94%, diglycerides 2.01%, monoglycerides 0.37% and 3.43% FFA. From the result of hydrolysis known that if FFA (free fatty acid) value is <1% and diglycerides is <0.45%, hydrolysis process will occur. The conclusion of the research that has been done are: R.Oryzae more effective than L.Plantarum for triglyceride hydrolysis process of NPLF nyamplung oil. The number of whole-cell microbial have no effect on the process. The length of time of hydrolysis would affect the levels of FFA (free fatty acid) which is formed because the longer the hydrolysis process can cause a reverse reaction or esterification reaction, so that triglyceride levels will incrise and the FFA will decrease.

Item Type: Thesis (Undergraduate)
Additional Information: RSK 660.284 2 Tah h
Uncontrolled Keywords: Nyamplung,Hidrolisis,Rhizopus oryzae, Lactobacillus plantarum, FFA, Non Polar Lipid Fraction, Whole-cell.
Subjects: T Technology > TP Chemical technology > TP248.3 Biochemical engineering. Bioprocess engineering
Divisions: Faculty of Industrial Technology > Chemical Engineering
Depositing User: ansi aflacha
Date Deposited: 05 Dec 2019 04:03
Last Modified: 05 Dec 2019 04:03
URI: http://repository.its.ac.id/id/eprint/72202

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