Emilisari, Martha (2015) Uji potensi khamir yang diisolasi dari kawasan mangrove Pantai Kenjeran Surabaya dalam mendegradasi selulosa. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Molekul selulosa merupakan polimer linier, bersifat
kristalin dan tidak mudah larut. Hal ini membuat selulosa tidak
mudah didegradasi secara kimia dan mekanis, sehingga
dibutuhkan kajian tentang penanganan degradasi selulosa secara
biologis dengan bantuan mikroorganisme. Salah satu
mikroorganisme potensial yang memiliki kemampuan dalam
mendegradasi selulosa adalah khamir. Penelitian ini bertujuan
untuk mengetahui genus khamir hasil isolasi dari mangrove
pantai Kenjeran Surabaya yang berpotensi dalam mendegradasi
selulosa, untuk mengetahui indeks hidrolisis selulosa tertinggi
dari khamir, serta kadar gula reduksi tertinggi dari khamir. Isolat
khamir yang diperoleh diidentifikasi hingga tingkat genus. Uji
potensi selulolitik khamir secara kualitatif dilakukan dengan
menggunakan medium CMC. Sementara, uji potensi secara
kuantitatif dilakukan dengan pengukuran kadar gula reduksi
dengan metode DNS pada isolat terbaik dari hasil uji kualitatif.
Hasil penelitian diperoleh lima isolat dengan kode K 1.2,
K 2.2, K 2.3, K 2.4, dan K 4.2. Isolat-isolat tersebut berpotensi
dalam mendegradasi selulosa. Nilai indeks selulolitik tertinggi
dicapai oleh isolat K 1.2 dengan nilai sebesar 0,725. Kadar gula
reduksi tertinggi yang dicapai oleh isolat K 1.2 adalah 0,190ppm
pada hari ketiga. ========== Cellulose is a linear polymer molecules, which is
crystalline and easily soluble. This makes the cellulose is not
easily degraded chemically and mechanically, so a study on the
cellulose degradation by biological treatment using
microorganisms is extremely needed. One of microorganisms
which have the ability to degrade cellulose is yeast. This study is
not only aims to determine the potential genus of yeasts to
degrade cellulose isolated from the mangrove areas of Kenjeran
Surabaya, but also to determine the highest index of yeast on
cellulose hydrolysis, and the highest sugar reduction level of
yeast. Yeast isolates were identified to the genus level. Test
potential cellulolytic yeasts qualitatively done using CMC
medium. Meanwhile, the potential for quantitative test is done by
measuring the levels of reducing sugars with the DNS method on
the best isolates from qualitative test results.
The results were obtained five isolates with the code K
1.2, K 2.2, K 2.3, K 2.4 and K 4.2. Isolates have the potential to
degrade cellulose. The highest index value cellulolitic achieved
by isolates K 1.2 with a value of 0.725. The highest reducing
sugar levels are achieved by isolates K 1.2 is 0,190 ppm on the
third day.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSBi 572.566 82 Emi u 3100015061194 |
| Uncontrolled Keywords: | khamir, selulosa, degradasi, gula reduksi, yeast, cellulose, degradation, reducing sugar |
| Subjects: | Q Science > QD Chemistry > QD320 Cellulose. Hydrolysis |
| Divisions: | Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | - Davi Wah |
| Date Deposited: | 09 Dec 2019 08:03 |
| Last Modified: | 09 Dec 2019 08:03 |
| URI: | http://repository.its.ac.id/id/eprint/72264 |
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