Rahmawati, Rizka (2016) Pengaruh Monosodium Glutamat (Msg) Komersial Terhadap Pertumbuhan Isolat Bakteri Bacillus Pl01 Dan Pseudomonas Pl01 Pada Mineral Salt Medium. Undergraduate thesis, Institut Teknologi Sepuluh Nopember Surabaya.
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Abstract
MSG merupakan hasil purifikasi glutamat sintesis yang
dapat menjadi makronutrient penyusun komponen seluler. Tujuan
dalam penelitian ini adalah untuk mengetahui pengaruh
pemberian MSG terhadap pertumbuhan isolat bakteri Bacillus
PL01 dan Pseudomonas PL01 pada MSM. Sebagai kontrol
digunakan medium Lactose Broth (LB). Pertumbuhan diukur
setiap 1 jam sekali selama 48 jam. Parameter yang diukur adalah
Optical Density (OD) λ 600nm, jumlah sel bakteri, serta
pengukuran diameter sel. Data dianalisis secara deskriptif
kuantitatif dengan Analysis of Variance (ANOVA).
Pada Bacillus PL01 pertumbuhan tertinggi terjadi
setelah 16 jam masa inkubasi pada konsentrasi 0,5 gr/L MSG
dengan nilai serapan OD 600nm yang setara dengan 3.93E+06
Sel/mL serta ukuran sel mencapai 1,8μm x 1,2μm, dimana
pertumbuhannya melebihi pertumbuhan di medium LB. Namun
terjadi penurunan pertumbuhan seiring dengan peningkatan
konsentrasi MSG. Pada Pseudomonas PL01 pertumbuhan
tertinggi setelah 16 jam masa inkubasi terjadi pada konsentrasi 2
gr/L MSG dengan nilai serapan OD 600nm yang setara dengan
9.97E+05 Sel/mL serta ukuran sel mencapai 2,4μm x 1,1μm.
Pertumbuhan Pseudomonas PL01 masih berada dibawah LB.
Berbanding terbalik dengan Bacillus PL01, pada Pseudomonas
v
PL01 semakin tinggi konsentrasi MSG, maka pertumbuhannya
semakin tinggi pula.
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MSG is a synthetic purified glutamate that can be used as
a nitrogen source for microorganisms growth. The purpose of this
research was to determine the effect of MSG on Bacillus PL01
and Pseudomonas PL01 growth in a Mineral Salt Medium. As a
control medium Lactose Broth (LB) was used. The bacterial
growth was measured every 1 hour for 48 hours
spectrophotometrically (OD 600nm), cells number using was
counted and cell shape was measured. Data were analyzed
statistically using a Quantitative with Analysis of Variance
(ANOVA).
In 0,5 gr/L MSG containing MSM, Bacillus PL01 growth
a highest speed after 16 hours it reached OD 600nm that equals to
3.93E+06 Cell/mL, and the cell size was around 1,8μm x 1,2μm.
This growth was even higher compared with it in LB. While
Pseudomonas PL01 reached the highest growth in 2 g/L MSG
containing MSM with OD 600nm that equals to 9.97E+05
Cell/mL with the cell size about 2,4μm x 1,1μm, but
Pseudomonas PL01 was more prefer to grow in LB at which its
growth was the best. The growth was increase over the MSG
concentration, even they were still lower than it in LB.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSB 664.4 Rah p-1 |
| Uncontrolled Keywords: | Bacillus PL01, MSG, MSM, Pertumbuhan Sel, Pseudomonas PL01 |
| Subjects: | Q Science > QR Microbiology > QR74.8 Bacteria |
| Divisions: | Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | EKO BUDI RAHARJO |
| Date Deposited: | 11 Dec 2019 08:51 |
| Last Modified: | 11 Dec 2019 08:51 |
| URI: | http://repository.its.ac.id/id/eprint/72329 |
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