Intani, Dwi Wahyu (2015) Aktivitas Enzim Ligninolitik Jamur Gliomastix Sp. Dan Mycelia Sterilia Sp. Dengan Induksi Logam Pada Limbah Bagase. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Bagase merupakan limbah pertanian lignoselulosa yang
dapat mendukung pertumbuhan mikroba untuk menghasilkan
enzim ligninolitik. Penggunaan konsorsium dan penambahan
logam-logam dasar diharapkan mampu meningkatkan produksi
enzim. Tujuan dari penelitian ini adalah untuk menguji pengaruh
Cu2+, Zn2+ dan Mn2+ terhadap aktivitas enzim ligninolitik jamur
Gliomastix sp.2 LM1020 dan Mycelia sterilia sp.2 LM1041 pada
limbah bagase.
Penelitian ini menggunakan metode fermentasi padat..
Variabel bebas adalah variasi kultur, dan konsentrasi logam
berat. Sedangkan variabel terikat adalah aktivitas enzim LiP,
MnP dan lakase. Data dianalisis dengan Anova dilanjutkan
dengan uji DMRT menggunakan Statistical Analysis System.
Hasil penelitian menunjukkan bahwa pada penambahan
Cu2+, Mycelia sterilia sp. menghasilkan aktivitas enzim
ligninolitik tertinggi yaitu LiP: 3433U/ml, MnP: 2237U/ml dan
lakase: 1345U/ml. Pada penambahan Zn2+, Gliomastix sp.
mempunyai aktivitas tertinggi yaitu LiP: 4910U/ml,
MnP:2285U/ml dan lakase:865U/ml. Pada penambahan Mn2+,
Gliomastix sp. juga mempunyai aktivitas enzim ligninolitik
tertinggi yaitu LiP :1130U/ml, MnP : 818U/ml, dan
lakase:1543U/ml.
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Bagasse is a lignocellulosic agricultural waste that can
support the growth of microbes to produce ligninolytic enzymes.
The Use of the consortium and the addition of base metals are
expected to increase the production of enzymes. The purpose of
this study was to examine the effect of Cu2+, Zn2+ and Mn2+ on the
activity of ligninolytic enzymes of Mycelia sterilia sp. LM1041
and Gliomastix sp. LM1020 on bagasse waste.
This study uses bagasse solid fermentation. The
independent variable is the variety of cultures, and the
concentration of heavy metals. The dependent variable is the
enzyme activity of LiP, MnP and laccase. Observed data analyzed
by Anova, continued by DMRT test with program Statistical
Analysis System.
The results showed that the addition of Cu2+, single
culture Mycelia sterilia sp. produces the highest activity, LiP at
3433U / ml, MnP 2237U / ml and laccase 1345U / ml. On the
addition of Zn2+, single culture Gliomastix sp. having the highest
activity, LiP at 4910U / ml, MnP 2285U / ml and laccase 865U /
ml. On the addition of Mn2+, single culture Gliomastix sp. also
had the highest activity, LiP at 1130U / ml, MnP of 818U / ml,
and laccase at 1543U / ml.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSBi 572.566 82 Int a |
| Uncontrolled Keywords: | Enzim ligninolitik, Gliomastix sp.2 LM1020, Logam, Mycelia sterilia sp.2 LM1041 |
| Subjects: | Q Science > QP Physiology > QP624 Molecular biology. |
| Divisions: | Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | Yeni Anita Gonti |
| Date Deposited: | 12 Feb 2020 03:55 |
| Last Modified: | 12 Feb 2020 03:55 |
| URI: | http://repository.its.ac.id/id/eprint/74828 |
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