Aktivitas Enzim Ligninolitik Jamur Gliomastix Sp. Dan Mycelia Sterilia Sp. Dengan Induksi Logam Pada Limbah Bagase

Intani, Dwi Wahyu (2015) Aktivitas Enzim Ligninolitik Jamur Gliomastix Sp. Dan Mycelia Sterilia Sp. Dengan Induksi Logam Pada Limbah Bagase. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Bagase merupakan limbah pertanian lignoselulosa yang dapat mendukung pertumbuhan mikroba untuk menghasilkan enzim ligninolitik. Penggunaan konsorsium dan penambahan logam-logam dasar diharapkan mampu meningkatkan produksi enzim. Tujuan dari penelitian ini adalah untuk menguji pengaruh Cu2+, Zn2+ dan Mn2+ terhadap aktivitas enzim ligninolitik jamur Gliomastix sp.2 LM1020 dan Mycelia sterilia sp.2 LM1041 pada limbah bagase. Penelitian ini menggunakan metode fermentasi padat.. Variabel bebas adalah variasi kultur, dan konsentrasi logam berat. Sedangkan variabel terikat adalah aktivitas enzim LiP, MnP dan lakase. Data dianalisis dengan Anova dilanjutkan dengan uji DMRT menggunakan Statistical Analysis System. Hasil penelitian menunjukkan bahwa pada penambahan Cu2+, Mycelia sterilia sp. menghasilkan aktivitas enzim ligninolitik tertinggi yaitu LiP: 3433U/ml, MnP: 2237U/ml dan lakase: 1345U/ml. Pada penambahan Zn2+, Gliomastix sp. mempunyai aktivitas tertinggi yaitu LiP: 4910U/ml, MnP:2285U/ml dan lakase:865U/ml. Pada penambahan Mn2+, Gliomastix sp. juga mempunyai aktivitas enzim ligninolitik tertinggi yaitu LiP :1130U/ml, MnP : 818U/ml, dan lakase:1543U/ml. =================================================================================================== Bagasse is a lignocellulosic agricultural waste that can support the growth of microbes to produce ligninolytic enzymes. The Use of the consortium and the addition of base metals are expected to increase the production of enzymes. The purpose of this study was to examine the effect of Cu2+, Zn2+ and Mn2+ on the activity of ligninolytic enzymes of Mycelia sterilia sp. LM1041 and Gliomastix sp. LM1020 on bagasse waste. This study uses bagasse solid fermentation. The independent variable is the variety of cultures, and the concentration of heavy metals. The dependent variable is the enzyme activity of LiP, MnP and laccase. Observed data analyzed by Anova, continued by DMRT test with program Statistical Analysis System. The results showed that the addition of Cu2+, single culture Mycelia sterilia sp. produces the highest activity, LiP at 3433U / ml, MnP 2237U / ml and laccase 1345U / ml. On the addition of Zn2+, single culture Gliomastix sp. having the highest activity, LiP at 4910U / ml, MnP 2285U / ml and laccase 865U / ml. On the addition of Mn2+, single culture Gliomastix sp. also had the highest activity, LiP at 1130U / ml, MnP of 818U / ml, and laccase at 1543U / ml.

Item Type: Thesis (Undergraduate)
Additional Information: RSBi 572.566 82 Int a
Uncontrolled Keywords: Enzim ligninolitik, Gliomastix sp.2 LM1020, Logam, Mycelia sterilia sp.2 LM1041
Subjects: Q Science > QP Physiology > QP624 Molecular biology.
Divisions: Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User: Yeni Anita Gonti
Date Deposited: 12 Feb 2020 03:55
Last Modified: 12 Feb 2020 03:55
URI: http://repository.its.ac.id/id/eprint/74828

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