Verdian, Trio (2015) Resistensi Dan Potensi Bacillus Sebagai Bioremoval Logam Kadmium (Cd). Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Keberadaan logam cadmium di lingkungan memiliki efek
toksik yang sangat tinggi. Cadmium tidak memiliki fungsi
biologis untuk organisme dan dapat menggangu metabolisme
secara seluler. Beberapa bakteri resisten terhadap logam
berat termasuk cadmium di antara dari genus Bacillus.
Apakah Bacillus yang resisten cadmium mampu melakukan
aktivitas bioremoval terhadap cadmium.
Masing-masing uji resistensi dan uji viabilitas dilakukan
pada media nutrien agar dan nutrien broth yang mengandung
CdCl2. Uji bioremoval dilakukan dengan pemaparan CdCl2 ke
dalam medium kultur Bacillus dan konsentrasi Cd diukur
dengan AAS. Analisis data diuji dengan ANOVA dan uji beda
nyata terkecil.
Semua isolat Bacillus resisten pada media yang
mengandung CdCl2 sampai dengan 30 mg/L. Isolat Bacillus
DA11 dapat mengurangi konsentrasi Cd2+ di medium
kultur paling besar dibanding isolat Bacillus S1 dan
SS19 yaitu 3,73 mg/L dari konsentrasi 23,75 mg/L yang
dipaparkan. Dengan efisiensi bioremoval maksimal
35,81% dari pemaparan 6,527 mg/L.
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Cadmium avalaibility at environtment causes
jeopardize effect for organism. Cadmium has no biological
function and disrupt cell metabolism. Bacillus is one of the
bacteria genera which resist to cadmium toxicity. Resistance
ability and Isolate viability tested using nutrien agar and
nutrient broth medium with various concentration of CdCl2.
Bioremoval efficiency assay by AAS and data analyzing with
ANOVA. The result shows that all of the isolate resisted to 30
mg/L CdCl2. Bacillus DA11 has the highest bioremoval
ability than S1 and SS19 at concentration 23,75 mg/L which
was 3,73 mg/L. It also has the highest bioremoval efficiency
at concentration 6,527 mg/L which was 35,81%.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSBi 579.362 Ver r |
| Uncontrolled Keywords: | Bacillus, Bioremoval, Kadmium, Logam Berat |
| Subjects: | Q Science > QH Biology > QH301 Biology |
| Divisions: | Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | Yeni Anita Gonti |
| Date Deposited: | 26 Feb 2020 08:34 |
| Last Modified: | 26 Feb 2020 08:34 |
| URI: | http://repository.its.ac.id/id/eprint/75200 |
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