Sari, Adisya Prima Nurmalita (2015) Resistensi Dan Potensi Bacillus Sebagai Bioremoval Logam Kromium (Cr). Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Kromium (Cr) tergolong logam berat yang dapat masuk ke
lingkungan melalui limbah industri sehingga mencemari
lingkungan. Bakteri resisten Cr mampu mengubah Cr(VI) menjadi
Cr(III). Penelitian ini bertujuan untuk mengetahui resistensi dan
potensi bioremoval kromium oleh Bacillus koleksi Laboratorium
Mikrobiologi dan Bioteknologi Jurusan Biologi ITS Surabaya.
Penelitian diawali dengan uji resistensi 6 isolat Bacillus untuk
mengetahui kemampuan tumbuh isolat pada medium yang
mengandung Cr. Dilakukan range finding test untuk mendapatkan
konsentrasi logam berat sebagai perlakuan bioremoval dan
menentukan 3 isolat terpilih. Kepadatan sel diukur dengan OD
menggunakan spektrofotometer UV-Vis dan divisualisasikan dengan
kurva pertumbuhan. Bioremoval Cr diukur dengan metode Atomic
Absorption Spectroscophy selanjutnya dilakukan uji viabilitas
dengan metode pour plate.
Hasil penelitian menunjukkan isolat Bacillus resisten dan viabel
terhadap logam Cr pada konsentrasi ≤ 150 mg/L. Pola pertumbuhan
isolat Bacillus yang tercekam logam Cr relatif lebih rendah
dibandingkan yang tidak tercekam Cr. Efisiensi bioremoval Cr yang
paling tinggi adalah Bacillus DA11 pada konsentrasi perlakuan
38.8 mg/L, sebesar 76.8%,
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Chromium (Cr) is one of heavy metal that encountered
environment from industrial waste so it can contaminate the
environment. Chromium resistance bacteria can transform Cr(VI) to
Cr(III). The aim of this research is to find out chromium resistance
and bioremoval potential of Microbiology and Biotechnology
Laboratory’s collection Bacillus spp.
This research was started with resistance assay of 6 isolates
Bacillus to determine growth ability of isolates on medium contained
Cr. Range finding test were done to determine heavy metal
concentration that used in research and determine 3 isolates that
more resistance than others. Cell density were measured by OD
using spectrophotometer UV-Vis and visualized by growt curve.
Chromium bioremoval was measured by Atomic Absorption
Spectroscophy method. Viability assay was done by pour plate
method.
The result showed that Bacillus resistant and viable on media
contained chromium at concentration ≤ 150 mg/L. Growth pattern of
Bacillus grown in media contained Cr was relative lower than
control. DA11 got highest bioremoval efficiency percentage in the
amount of 76.8% at concentration 38.8 mg/L
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSBi 579.362 Sar r |
| Uncontrolled Keywords: | Bacillus, bioremoval, Chromium (Cr), resistensi |
| Subjects: | Q Science > QH Biology > QH301 Biology |
| Divisions: | Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | Yeni Anita Gonti |
| Date Deposited: | 26 Feb 2020 08:49 |
| Last Modified: | 26 Feb 2020 08:49 |
| URI: | http://repository.its.ac.id/id/eprint/75201 |
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