Puspita, Putri Suryaning (2020) Studi Pengaruh Ozon (O3) Terhadap Reduksi S. Aureus Biofilm Menggunakan Photosensitizer Curcuma L. Extract. Masters thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Staphylococcus aureus (S. aureus) adalah flora normal yang disebabkan infeksi kulit ringan dan memiliki kemampuan membentuk biofilm. Biofilm adalah komunitas mikroba yang melekat pada permukaan dan terbungkus dalam matriks mikroba ekstraseluler. Tujuan dari penelitian ini adalah untuk meningkatkan efek antibakteri dari terapi fotodinamik menggunakan paparan ozon untuk mengurangi biofilm S. aureus. Terapi fotodinamik (PDT) adalah proses terapi, yang melibatkan kombinasi agen cahaya dan fotosensitizer. Ekstrak kurkumin dari Curcuma longa (C. longa) sebagai agen fotosensitizer dan sumber cahaya yang sesuai. Ekstrak curcumin dibuat dari Merck (Jerman) dan dilarutkan dalam etanol 10 mg / mL. Larutan diencerkan hingga mencapai konsentrasi 6,75 x 10-5 M. Untuk menentukan efek antibakteri dari perawatan pada biofilm S. aureus, sampel didistribusikan ke dalam 7 kelompok: C, PL, CL, PO, CO, PLO dan CLO. Pertumbuhan bakteri dilihat dengan mengukur optical density (OD) pada 595nm dengan pembaca ELISA. Perbedaan reduksi tertinggi antara perlakuan PL dan CL terjadi pada detik ke-30 dengan reduksi masing-masing 65,55% dan 85,63%. Ozon yang ditambahkan pada photosensitizer endogen (PO) dan eksogen (CO) memberikan efek reduksi tertinggi masing-masing sebesar 30,74% dan 79,38% dengan exposure selama 30 detik. Pemberian ozon selama 50 detik mampu mereduksi sel tertinggi sebanyak 97,63% (PLO) dan 94,55% (CLO). Hasil ini menunjukkan bahwa penambahan curcumin dengan exposure ozon terlama memberikan efek reduksi sel tertinggi dibanding perlakuan sebelumnya. Kombinasi PDT dan ozon gas memberikan peningkatan reduksi biofilm dibandingkan dengan PDT.
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Staphylococcus aureus (S. aureus) is a normal flora caused mild skin infections and has the ability to form biofilms. Biofilms are microbial communities attached to surfaces and encased in an extracellular matrix of microbial. The aim of this study was to improve the antibacterial effect of photodynamic therapy using ozone exposure for reducing the S. aureus biofilms. Photodynamic therapy (PDT) is a therapeutic process, involving the combination of light and photosensitizers agents. Curcumin extract from Curcuma longa (C. longa) as a photosensitizer agent and the appropriate light source. The curcumin extract were prepared from Merck (Germany) and dissolved in ethanol 10 mg/mL. The solution is diluted until it reaches concentration 6.75 x 10-5 M. To determine the antibacterial effect of treatments on S. aureus biofilms, samples were distributed into 7 groups: C, PL, CL, PO, CO, PLO and CLO. The growth of bacteria had been monitored by measuring the optical density (OD) at 595nm with ELISA reader. The highest reduction effect between PL and CL treatment occur at 30 seconds with reduction of 65,55% and 85,63%, respectively. Ozone added to the endogenous photosensitizer (PO) and exogenous (CO) has the highest reduction effect of 30.74% and 79.38% respectively with an exposure of 30 seconds. Ozone added for 50 seconds was able to reduce the highest cells of 97.63% (PLO) and 94.55% (CLO). These results indicate that the addition of curcumin with the longest ozone exposure gives the highest cell reduction effect compared to the previous treatment. The combination of PDT and gaseous ozone provides to increase of biofilms reduction compared to the PDT.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | RTF 579.17 Pus s-1 |
| Uncontrolled Keywords: | Biofilm, Kurkumin, Ozon, PDT, Reduksi |
| Subjects: | Q Science Q Science > QC Physics > QC162 Adsorption and absorption Q Science > QC Physics > QC271 Temperature measurements Q Science > QC Physics > QC451 Spectroscopy Q Science > QD Chemistry > QD117 Absorption Q Science > QD Chemistry > QD320 Cellulose. Hydrolysis Q Science > QD Chemistry > QD471 Chemical compounds - Structure and formulas Q Science > QD Chemistry > QD96F56 Fluorescence spectroscopy Q Science > QH Biology > QH541.15.T68 Toxicity testing Q Science > QR Microbiology > QR74.8 Bacteria |
| Divisions: | Faculty of Industrial Technology > Physics Engineering > 30101-(S2) Master Thesis |
| Depositing User: | Putri Suryaning Puspita |
| Date Deposited: | 07 Mar 2025 00:51 |
| Last Modified: | 07 Mar 2025 00:51 |
| URI: | http://repository.its.ac.id/id/eprint/75454 |
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