Ulfi, Aulia (2016) Pengaruh Penambahan Bakteri Terhadap Biodegradasi Ddt Oleh Gloeophyllum Trabeum. Masters thesis, Institut Teknologi Sepuluh Nopember Surabaya.
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Abstract
DDT (1,1,1-trikloro-2,2 bis (4-klorofenil) etana) merupakan insektisida
sintesis yang memiliki beberapa efek negatif bagi lingkungan dan manusia. Oleh
karena itu, sangat penting untuk menentukan metode yang efektif untuk degradasi.
Jamur pelapuk coklat, Gloeophyllum trabeum mempunyai kemampuan untuk
mendegradasi DDT, namun kemampuannya membutuhkan waktu yang sangat
lama. Pada penelitian ini, pengaruh penambahan Bacillus subtilis, Pseudomonas
aeruginosa dan Ralstonia pickettii (screening bakteri), variasi jumlah penambahan
bakteri 1, 3, 5, 7 dan 10 mL dan variasi waktu penambahan bakteri pada hari ke 0,
1, 3, dan 5 terhadap degradasi DDT oleh G. trabeum telah diuji. R. pickettii dapat
meningkatkan proses degradasi, dimana penambahan R. pickettii 10 mL pada hari
ke-1 menunjukkan biodegradasi DDT paling tinggi yaitu 92.41% selama 7 hari
inkubasi. DDD terdeteksi sebagai metabolit produk melalui reaksi deklorinasi
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DDT (1,1,1-trichloro-2,2 bis (4-chlorophenyl) ethane) is a synthetic
insecticide which has several negative effects to the environment and human.
Therefore, determining an effective method to reduce DDT may give beneficial
impact. Brown-rot fungus, Gloeophyllum trabeum, is well known to have an
ability to degrade DDT, despite the fact that it might require a long-term
remediation. In this study, the effect of addition of Bacillus subtilis, Pseudomonas
aeruginosa and Ralstonia pickettii (screening of bacteria), the volume of bacteria
at 1, 3, 5, and 10 mL and biodegradation the time range of addition of bacteria on
days 0, 1, 3, and 5 on biodegradation of DDT by G. trabeum had been
investigated. R. pickettii enhanced the degradation process, in which the addition
of 10 ml of this bacteria at day 1 possessed a highest value of 92.41% within 7
days incubation. DDD was detected to be a product metabolite through
dechlorination reaction
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | RTKi 579.332 Ulf p |
| Uncontrolled Keywords: | Biodegradasi, DDT, Bacillus subtilis, Pseudomonas aeruginosa, Ralstonia pickettii, Gloeophyllum trabeum. |
| Subjects: | Q Science > QR Microbiology > QR74.8 Bacteria |
| Divisions: | Faculty of Mathematics and Science > Chemistry > 47101-(S2) Master Thesis |
| Depositing User: | EKO BUDI RAHARJO |
| Date Deposited: | 03 Jul 2020 04:01 |
| Last Modified: | 03 Jul 2020 04:01 |
| URI: | http://repository.its.ac.id/id/eprint/76293 |
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