Pengaruh Penambahan Bakteri Terhadap Biodegradasi Ddt Oleh Gloeophyllum Trabeum

Ulfi, Aulia (2016) Pengaruh Penambahan Bakteri Terhadap Biodegradasi Ddt Oleh Gloeophyllum Trabeum. Masters thesis, Institut Teknologi Sepuluh Nopember Surabaya.

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Abstract

DDT (1,1,1-trikloro-2,2 bis (4-klorofenil) etana) merupakan insektisida sintesis yang memiliki beberapa efek negatif bagi lingkungan dan manusia. Oleh karena itu, sangat penting untuk menentukan metode yang efektif untuk degradasi. Jamur pelapuk coklat, Gloeophyllum trabeum mempunyai kemampuan untuk mendegradasi DDT, namun kemampuannya membutuhkan waktu yang sangat lama. Pada penelitian ini, pengaruh penambahan Bacillus subtilis, Pseudomonas aeruginosa dan Ralstonia pickettii (screening bakteri), variasi jumlah penambahan bakteri 1, 3, 5, 7 dan 10 mL dan variasi waktu penambahan bakteri pada hari ke 0, 1, 3, dan 5 terhadap degradasi DDT oleh G. trabeum telah diuji. R. pickettii dapat meningkatkan proses degradasi, dimana penambahan R. pickettii 10 mL pada hari ke-1 menunjukkan biodegradasi DDT paling tinggi yaitu 92.41% selama 7 hari inkubasi. DDD terdeteksi sebagai metabolit produk melalui reaksi deklorinasi ============================================================================================ DDT (1,1,1-trichloro-2,2 bis (4-chlorophenyl) ethane) is a synthetic insecticide which has several negative effects to the environment and human. Therefore, determining an effective method to reduce DDT may give beneficial impact. Brown-rot fungus, Gloeophyllum trabeum, is well known to have an ability to degrade DDT, despite the fact that it might require a long-term remediation. In this study, the effect of addition of Bacillus subtilis, Pseudomonas aeruginosa and Ralstonia pickettii (screening of bacteria), the volume of bacteria at 1, 3, 5, and 10 mL and biodegradation the time range of addition of bacteria on days 0, 1, 3, and 5 on biodegradation of DDT by G. trabeum had been investigated. R. pickettii enhanced the degradation process, in which the addition of 10 ml of this bacteria at day 1 possessed a highest value of 92.41% within 7 days incubation. DDD was detected to be a product metabolite through dechlorination reaction

Item Type: Thesis (Masters)
Additional Information: RTKi 579.332 Ulf p
Uncontrolled Keywords: Biodegradasi, DDT, Bacillus subtilis, Pseudomonas aeruginosa, Ralstonia pickettii, Gloeophyllum trabeum.
Subjects: Q Science > QR Microbiology > QR74.8 Bacteria
Divisions: Faculty of Mathematics and Science > Chemistry > 47101-(S2) Master Thesis
Depositing User: EKO BUDI RAHARJO
Date Deposited: 03 Jul 2020 04:01
Last Modified: 03 Jul 2020 04:01
URI: http://repository.its.ac.id/id/eprint/76293

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