Himayani, Chusnul Eka Safitri (2020) Keragaman Genetik dan Respon Morfologi Beberapa Varietas Tomat (Solanum lycopersicum) Serta Kajian Literatur Respon Fisiologi Dan Molekuler Tanaman Terhadap Cekaman Logam Berat Pb. Masters thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Tomat (Solanum lycopersicum) merupakan sayuran buah yang memiliki potensi di bidang industri pangan, kosmetik dan kesehatan. Hal ini disebabkan karena kandungan senyawa karotenoid (likopen) yang tinggi, memiliki aktivitas antioksidan, anti kanker, vitamin dan mineral. S. lycopersicum memiliki beberapa varietas diantaranya varietas Mutiara, Rewakto F1, Permata F1 dan Opal. Produktivitas varietas S. lycopersicum dipengaruhi oleh banyak faktor lingkungan. Salah satunya adalah paparan logam berat pada tanah. Logam berat yang sering ditemukan di lingkungan adalah logam berat Pb. Tanaman memiliki mekanisme respon yang bervariatif terhadap logam berat karena disebabkan oleh faktor genotip. Penelitian ini bertujuan untuk mengetahui respon morfo-fisiologi dan molekular tanaman tomat terhadap cekaman Pb dan untuk mengetahui keragaman genetik beberapa varietas tanaman tomat. Penelitian dilakukan 2 aspek penelitian di laboratorium dan aspek studi literatur. Aspek penelitian meliputi keragaman genetik, respon morfologi yang diamati adalah panjang akar, tinggi tanaman, serta dianalisa dengan Anova two way menggunakan Rancangan Acak Lengkap (RAL) faktorial dengan dua faktor (varietas tomat (Mutiara, Rewako F1, Permata F1 dan Opal) dan konsentrasi Pb (0, 75, 150 dan 300 ppm). Aspek studi literatur meliputi kadar prolin, kandungan klorofil, dan molekular yang akan dianalisa secara deskriptif kuantitatif. Marka RAPD dapat menunjukkan keragaman dari 4 varietas tomat. Dalam penelitian dan studi literatur ini logam berat Pb dapat menurunkan panjang akar, kadar prolin, kadar klorofil dan mempengaruhi ekspresi gen yang terlibat dalam protein folding (LEMT-1, LEMT-2, LEMT-3, LEMT-4, LEHsp90-1), biosintesis fitohormon (NCED 2/3, PIN1, EIN 2) dan metabolit antioksidan (P5CS1, GSH).
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Tomato (Solanum lycopersicum) is a fruit vegetable that has potential in the food, cosmetics and health industries. This is due to the high content of carotenoid (lycopene) compounds, which have antioxidant, anti-cancer, vitamin and mineral activities. S. lycopersicum has several varieties including Pearl varieties, Rewakto F1, Permata F1 and Opal. Productivity of S. lycopersicum is influenced by many environmental factors. One of them is exposure to heavy metals in the soil. Heavy metals that are often found in the environment are heavy metals Pb. Plants have varied response mechanisms to heavy metals because they are caused by genotypic factors. This study aims to determine the morpho-physiological and molecular responses of tomato plants to the Pb stress and to determine the genetic diversity of several varieties of tomato plants. The study was conducted 2 aspects of research in the laboratory and aspects of literature studies. Research aspects include genetic diversity, the observed morphological responses are root length, plant height, and analyzed by Anova two way using a factorial completely randomized design with two factors (tomato varieties (Mutiara, Rewako F1, Permata F1 and Opal) and concentration Pb (0, 75, 150 and 300 ppm) Aspects of the literature study include proline levels, chlorophyll content, and molecular content to be analyzed descriptively quantitatively. RAPD markers can show the diversity of 4 varieties of tomatoes.In this research and literature studies the heavy metals Pb can reduce root length, proline levels, chlorophyll levels and affect gene modulation in protein folding (LEMT-1, LEMT-2, LEMT-3, LEMT-4, LEHsp90-1), phytohormone biosynthesis (NCED 2/3, PIN1, EIN 2) and antioxidant metabolites (P5CS1, GSH).
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | ekspresi gen, logam berat Pb, keragaman genetik, RAPD, Solanum lycopersium. genetic expression, genetic diversity, heavy Metals Pb, RAPD, Solanum lycopersicum. |
| Subjects: | Q Science > QK Botany |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46101-(S2) Master Thesis |
| Depositing User: | Chusnul Eka Safitri Himayani |
| Date Deposited: | 03 Aug 2020 07:49 |
| Last Modified: | 03 Aug 2020 07:49 |
| URI: | http://repository.its.ac.id/id/eprint/76788 |
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