Ainiya, Sidratu (2014) Biogrouting: Produksi Urease Dari Bakteri Laut (Oceanobacillus Sp.) Pengendap Karbonat. Undergraduate thesis, Institut Teknologi Sepuluh Nopember Surabaya.
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Abstract
Biogrouting adalah teknologi yang mensimulasikan
proses diagenesis yaitu transformasi butiran pasir menjadi
batuan pasir (calcarinite/sandstone). Permasalahan dalam
penelitian ini adalah bagaimana mengoptimasi produk urease
dengan melakukan uji aktifitas, mengisolasi, mempurifikasi dan
mengkarakterisasi urease serta mengaplikasikannya sebagai
material grout. Uji aktifitas dan optimasi dilakukan dengan
menumbuhkan isolat Oceanobacillus sp. pada 2 variasi medium
(B4 urea dan B4 urin), 5 variasi pH (4-8) dan 2 variasi suhu
(25°C dan 29°C). Hasil uji aktifitas dan optimasi selanjutnya
dipurifikasi menggunakan ammonium sulfat (Uji Bradford) dan
dicari titik isoelektriknya. Kemudian hasil protein presipitat
dikarakterisasi menggunakan SDS-PAGE. Berdasarkan hasil
penelitian diketahui bahwa aktifitas urease paling tinggi adalah
70.21 unit/ml. Urease optimal dihasilkan pada isolat yang
ditumbuhkan pada B4 urea pada pH 7 temperatur 25°C. Berat
molekul urease yang dikarakterisasi menggunakan SDS-PAGE
adalah 440 kDa, sedangkan titik isoelektriknya pada pH 6.
Urease dapat dijadikan material grout karena memiliki
kemampuan untuk melakukan sementasi pada aplikasi sederhana
biogrouting
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Grouting is the process of pore filling with grout
material (construction material). Biogrouting is a technology that
simulates the process of diagenesis, namely the transformation of
grains of sand into sandstone (calcarinite / sandstone). Calcite
(CaCO ₃) formed from biogrouting process would bind sand
grains causing the cementation process. The research problem is
how to optimize the product with the urease activity test, isolate,
purify and characterize urease and applying it as a grout material.
Testing and optimization activities carried out by growing isolates
Oceanobacillus sp. 2 variations on the medium (urea and B4 B4
urine), 5 variations of pH (4-8) and 2 variations of temperature
(25 ° C and 29 ° C). Test results and optimization activities
further purified using ammonium sulfate (Test Bradford) and
sought isoelectric point. Then the results of the protein
precipitates were characterized using SDS-PAGE. Based on the
survey results revealed that the highest urease activity was 70.21
units / ml (generated from isolates of urease on urea medium B4).
Optimal urease produced in isolates grown in medium B4 urine
pH 8 and a temperature of 25 ° C, while in medium B4 urea at pH
7 a temperature of 25 ° C. The molecular weight of urease were
characterized using SDS-PAGE was 440 kDa, whereas the
isoelectric point at pH 6. Urease can be used as grouting material
because it has the ability to do a simple application biogrouting
cementation.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSBi 552.58 Ain b |
| Uncontrolled Keywords: | Biogrouting, diagenesis, Oceanobacillus sp., urease |
| Subjects: | Q Science > QR Microbiology > QR74.8 Bacteria |
| Divisions: | Faculty of Natural Science > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | EKO BUDI RAHARJO |
| Date Deposited: | 11 Sep 2020 00:19 |
| Last Modified: | 11 Sep 2020 00:19 |
| URI: | http://repository.its.ac.id/id/eprint/81856 |
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