Khotimah, Kusnul (2014) Azotobacter Sebagai Bioakumulator Merkuri. Undergraduate thesis, Institut Teknologi Sepuluh Nopember Surabaya.
Preview |
Text
1510100043-Undergraduate_Thesis.pdf - Published Version Download (1MB) | Preview |
Abstract
Merkuri merupakan logam berat paling toksik
dibandingkan dengan logam berat lainnya. Beberapa bakteri ada
yang resisten merkuri. Salah satu genus bakteri resisten merkuri
dan mampu mengakumulasi merkuri yaitu Azotobacter.
Azotobacter merupakan bakteri pemfiksasi nitrogen bebas non
simbiotik yang melimpah di daerah rhizosfer lahan pertanian dan
merupakan bakteri penghasil EPS yang dapat berfungsi sebagai
pengkhelat logam.
Penelitian ini bertujuan untuk mendapatkan isolat
Azotobacter yang resisten terhadap merkuri HgCl2, dan
mengukur kemampuan bioakumulasinya terhadap HgCl2. Isolasi
bakteri Azotobacter dilakukan dengan media selektif Azotobacter.
uji resistensi HgCl2 dilakukan dengan streak agar miring dan
kemampuan bioakumulasi diukur dengan metode serapan atom
serta uji viabilitas menggunakan metode pour plate. Analisis
beda nyata dengan ANOVA pada taraf 5% dilanjutkan dengan uji
beda nyata terkecil (BNT).
Tiga isolat Azotobacter dari lahan eco urban farming ITS
resisten terhadap HgCl2 sampai 20 mg/L yaitu A5, A6, dan A9.
Efisiensi bioakumulasi yang tertinggi pada pemaparan HgCl2 5
mg/L yaitu isolat A5 (89%) dan A9 (87%)
================================================================================================
Mercury is the most toxic heavy metals compared with
other heavy metals. Some bacteria are resistant to mercury. One
of mercury resistant bacteria genus and able to accumulate of
mercury is Azotobacter. Azotobacter is free-living nitrogen fixing
bacteria which abundant in rhizosfer area of agricultural land and
it is EPS producing bacteria that can serve as chelating metals.
This research aims to get Azotobacter isolates resistant to
mercury HgCl2, and measure the ability of mercury
biaccumulation. Isolation of Azotobacter by selective Azotobacter
media, resistance test by streak at slant agar, and bioaccumulation
ability measured by atomic absorption method and vaibility test
using pour plate method. Observed data analyzed by ANOVA,
continued by Least Significant Difference (LSD) test, Both test at
level 5 %.
Three Azotobacter isolates from eco urban farming ITS
land are resistant until 20 mg/L of HgCl2 which is A5, A6 and
A9. The highest efficiency bioaccumlation on exposure 5 mg/L
HgCl2 is A5 (89%) and A9 (87%)
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSBi 571.954 663 Kho a |
| Uncontrolled Keywords: | Azotobacter, bioakumulator, merkuri, uji resistensi |
| Subjects: | Q Science > QH Biology > QH541.15.T68 Toxicity testing |
| Divisions: | Faculty of Natural Science > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | EKO BUDI RAHARJO |
| Date Deposited: | 11 Sep 2020 01:08 |
| Last Modified: | 11 Sep 2020 01:08 |
| URI: | http://repository.its.ac.id/id/eprint/81858 |
Actions (login required)
 |
View Item |