Kurniawati, Alfia Rahma (2014) Pengaruh Medium Limbah Organik Terhadap Aktivitas Enzim Lipase Dari Isolat Kapang Tanah Wonorejo. Undergraduate thesis, Institut Teknologi Sepuluh Nopember Surabaya.
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Abstract
Meningkatnya akumulasi limbah cair organik yang mengandung lipid di lingkungan perlu ditanggulangi. Penelitian ini bertujuan untuk mengetahui aktivitas enzim yang dihasilkan dari isolat kapang Mortierella sp. T3.G1, Aspergillus niger T2.1, Penicillium sp.1 T4.E3 secara tunggal dan konsorsium dalam mendegradasi lipid pada medium limbah organik yang berbeda.
Parameter yang diamati dalam penelitian ini adalah nilai degradasi lipid dan nilai aktivitas enzim lipase. Limbah cair organik yang digunakan adalah limbah cair pencucian ikan, limbah cair tangki septik dan limbah minyak goreng bekas. Data pengaruh medium limbah cair organik terhadap aktivitas enzim lipase dianalisis dengan menggunakan ANOVA oneway dan dilanjutkan dengan uji Duncan.
Hasil menunjukkan bahwa medium dan isolat berpengaruh terhadap aktivitas lipase dengan nilai P=0,000. Korelasi nilai aktivitas dan nilai degradasi tertinggi ditunjukkan pada penambahan isolat Aspergillus niger dengan nilai aktivitas lipase sebesar 0,1511 U/mL dan degradasi lipid 83,95% pada limbah minyak goreng bekas, 0,0997 U/mL dan degradasi lipid 58,43% pada limbah pencucian ikan dan aktivitas lipase 0,0697 U/mL dan degradasi lipid 67,36% pada limbah tangki septik
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Increasing the accumulation of organic waste liquid that
containing lipids in the neighborhood need to overcome. This
research is aimed to know the activity of an enzyme lipase from
mold isolate Mortierella sp. T3.G1, Aspergillus niger T2.1, and
Penicillium sp.1 T4.E3 singly and a consortium on different
medium liquid waste organic.
The parameters that are tested in this research is the
degradation of lipid and the activity of an enzyme lipase. Organic
waste liquid that used there are processing fish wastewater,septic
tank wastewater and used cooking waste oil. The influence data
of medium against the activity of an enzyme protease analyzed
using Annova One-Way and continued with Duncan test.
The result showed that medium and isolates affecting the
activity of enzymes lipase with P value=0,000. Correlation of
activity value and degradation value indicated that isolate
Aspergillus niger is the highest in three organic waste liquid with
activity lipase 0,0997 U/mL and degradation lipid 58,43 % on
processing fish wastewater, activity lipase 0,1511 U/mL and
degradation lipid 83,95 % on used cooking waste oil and lipase
0,0697 U/mL and degradation lipid 67,36 % on septic tank
wastewater
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSBi 660.634 Kur p |
| Uncontrolled Keywords: | Limbah Organik, Lipase, Degradasi Lipid |
| Subjects: | Q Science > QP Physiology > QP609.P78 Proteolytic enzymes. T Technology > TD Environmental technology. Sanitary engineering > TD898.8.C67 Waste disposal |
| Divisions: | Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | EKO BUDI RAHARJO |
| Date Deposited: | 21 Oct 2020 01:43 |
| Last Modified: | 21 Oct 2020 01:43 |
| URI: | http://repository.its.ac.id/id/eprint/82170 |
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