Meitamasari, Anindita (2014) Uji Kemampuan Mixed Culture Bakteri Bacillus Subtilis Dan Bacillus Megaterium Untuk Meremoval Logam Berat Kromium (Iii). Undergraduate thesis, Institut Teknologi Sepuluh Nopember Surabaya.
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Abstract
Faktor pencemar yang sering ditemukan dalam perairan
adalah logam berat merkuri, cadmium, kromium, dan plumbum
yang dapat mempengaruhi kesehatan manusia bila bersentuhan
langsung atau mengkonsumsi air tersebut. Logam berat pada air
akan semakin meningkat dengan adanya akumulasi dari air
limbah yang dibuang ke sungai. Penelitian ini merupakan upaya
untuk memperbaiki keadaan lingkungan dengan cara biosorpsi
bakteri Bacillus subtilis dan Bacillus megaterium yang akan
dilihat keefektifannya dalam mereduksi logam berat dengan cara
mixed culture dengan perbandingan 25:75, 50:50 dan 75:25.
Tiap perbandingan mixed culture bakteri dianalisa
kemampuan removal terhadap logam berat kromium dengan
konsentrasi berbeda yaitu 50 mg/L, 75 mg/L dan 100 mg/L.
Analisis juga dilakukan pada perlakuan salinitas dan tanpa
salinitas. Pertumbuhan bakteri akan dianalisa setiap 2 jam dengan
menggunakan Optical Density (OD) pada panjang gelombang
600 nm dan analisa kromium saat awal, tengah dan akhir
penelitian dengan menggunakan metode AAS.
Hasil penelitian ini menunjukan mixed culture bakteri
yang efektif dalam meremoval kromium adalah pada perlakuan
salinitas yang mampu meremoval hingga 66,23% pada mixed
culture bakteri dengan perbandingan 25:75 dan pada konsentrasi
200 mg/L dalam waktu 3 jam
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The most frequently factors that found as contaminants is
heavy metals such as mercury, cadmium, chromium and
plumbum that may affect human health when consume the water.
Existence of heavy metals in the water will rise to the
accumulation of waste water discharged into the river
continously. This research is an attempt to improve environment
with biosorption using bacteria Bacillus subtilis and Bacillus
megaterium which will see the effectiveness to reduce the heavy
metals with mixed culture at a ratio 25:75, 50:50 and 75:25.
Every mixed culture bacteria ratio analyzed the ability of
removal of the chromium with different concentration 50 mg/L,
75 mg/L and 100 mg/L. The analysis was also performed on
salinity and without salinity. Bacterial growth will be analyzed
every 2 hours using Optical Density (OD) at a wavelength 600
nm and analysis of chromium using AAS method at the end and
beginning.
The results of this research showed mixed culture of
bacteria that are effective in removed of chromium in the
wastewater is mixed culture with ratio 25:75 with a 66,23% rate
of effectiveness to removal chromium concentration of 200 mg/L
with salinity treatment for 3 hours
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSL 628.167 42 Mei u |
| Uncontrolled Keywords: | Biosorpsi, Bacillus subtilis, Bacillus megaterium, Kromium, Mixed Culture |
| Subjects: | T Technology > TD Environmental technology. Sanitary engineering > TD646 Sewage--Purification |
| Divisions: | Faculty of Civil Engineering and Planning > Environment Engineering > 25201-(S1) Undergraduate Thesis |
| Depositing User: | EKO BUDI RAHARJO |
| Date Deposited: | 26 Nov 2020 06:57 |
| Last Modified: | 26 Nov 2020 06:57 |
| URI: | http://repository.its.ac.id/id/eprint/82280 |
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