Aktivitas α-glukosidase dari γ-mangostin dan Turunannya pada Kulit Buah Manggis

Rafsanjani, Ega Rocky Maulana (2021) Aktivitas α-glukosidase dari γ-mangostin dan Turunannya pada Kulit Buah Manggis. Masters thesis, Institut Teknologi Srpuluh Nopember.

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Abstract

Manggis (Garcinia mangostana Linn) dilaporkan kaya akan turunan senyawa santon teroksigenasi dan terprenilasi, seperti γ-mangostin (1) yang memberikan efek farmakologi antiinflamasi, antikanker, dan antidiabetes. Produk modifikasi turunan senyawa santon dengan pereaksi asetat anhidrat mampu meningkatkan aktivitas antidiabetes. Modifikasi isolat α- dan β-mangostin dengan asetat anhidrat menghasilkan turunan metilester tersubsitusi pada isolat mangostin. Hal ini menjadi dasar mendapatkan produk turunan 3,6,7-tri-metilester-γ-mangostin dari isolat mangostin. Dua isolat mangostin (1) dan α-mangostin (2) dari kulit buah manggis, diperoleh melalui proses maserasi menggunakan pelarut etil asetat, difraksinasi menggunakan metoda kromatografi Cair Vakum (KCV) dengan fasa diam Silika Gel serta dielusi menggunakan eluen yang ditingkatkan kepolarannya, sampai dihasilkan dua isolat murni yakni isolat (1) sebanyak 1,43 gram dan isolat (2) sebanyak 3,13 gram. Sedangkan produk modifikasi dari mangostin (1), yaitu 3,6,7-tri-metilester-γ-mangostin (3) dihasilkan 32,9 mg. Penentuan struktur isolat dan produk modifikasi memanfaatkan data spektroskopi UV, IR, NMR, dan HRESIMS. Hasil uji persentase (%) penghambatan enzim α-glukosidase menunjukkan data berturut-turut dengan nilai: IC50 9,79 μM untuk γ-mangostin (1), IC50 55,17 μM α-mangostin (2) dan IC50 2,24 μM untuk 3,6,7-tri-metilester-γ-mangostin (3) dengan kontrol positif akarbosa IC50 4,55 μM.
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Mangosteen (Garcinia mangostana Linn) is reported to have many derivatives of oxygenated and prenylated xanthones such as γ-mangostin which provide pharmacological effects such as antiinflammatory, anticancer, and antidiabetic. Modified products of xanthones derivatives using anhydrous acetate reagent were able to increase antidiabetic activity. Modification of α- and β-mangostin isolate using anhydrous acetate produced substituted methylester derivatives in mangostin isolates. This is a reference for obtaining a derivative product of 3,6,7-tri-methylester-γ-mangostin from γ-mangostin isolate. Two isolates of γ-mangostin (1) and α-mangostin (2) from mangosteen pericarp, were obtained through a maceration process using ethyl acetate solvent, fractionated using the Vacuum Liquid Chromatography (KCV) method with Silica Gel as a stationary phase and eluted using an eluent that was increased in polarity. , until two pure isolates were produced, namely isolate (1) as much as 1.43 grams and isolate (2) as much as 3.13 grams. While the modified product of γ-mangostin (1), namely 3,6,7-tri-methylester-γ-mangostin (3) produced 32.9 mg. Determination of the structure of isolates and modified products made use of UV, IR, NMR, and HRESIMS spectroscopy data. The results of the percentage (%) inhibition of the α-glucosidase enzyme showed successive data with values: IC50 9,79 μM for γ-mangostin (1), IC50 55,17 μM α-mangostin (2) and IC50 2,24 μM for 3.6, 7-tri-methylester-γ-mangostin (3) with a positive control of acarbose IC50 4.55 μM.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	Garcinia mangostana Linn, α-mangostin, γ-mangostin, 3,6,7 tri metilester-γ-mangostin
Subjects:	Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry Q Science > QD Chemistry > QD63 Extraction
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47101-(S2) Master Thesis
Depositing User:	Ega Rocky Maulana Rafsanjani
Date Deposited:	05 Mar 2021 03:43
Last Modified:	05 Mar 2021 03:43
URI:	http://repository.its.ac.id/id/eprint/83531

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