Pengaruh Kandungan Sukrosa Pada Medium Dan Fotoperiode Terhadap Pertumbuhan Benih Sintetik Stevia Rebaudiana Bertoni

Sholihah, May Fiatus (2021) Pengaruh Kandungan Sukrosa Pada Medium Dan Fotoperiode Terhadap Pertumbuhan Benih Sintetik Stevia Rebaudiana Bertoni. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Teknik perbanyakan Stevia rebaudiana secara in vitro salah satunya yaitu menggunakan teknologi benih sintetik. Benih sintetik diartikan sebagai eksplan yang dienkapsulasi seperti embrio somatik, pucuk, tunas, sel agregat atau jaringan lain yang memiliki kemampuan untuk berkembang menjadi tanaman dalam kondisi in vitro atau ex vitro dan dapat mempertahankan potensinya setelah penyimpanan. Penelitian ini bertujuan untuk mengetahui pengaruh kandungan sukrosa dan fotoperiode terhadap pertumbuhan benih sintetik Stevia rebaudiana Bertoni. Bahan tanam yang digunakan berupa pucuk tanaman Stevia rebaudiana yang dienkapsulasi menggunakan 4% natrium alginat dan 75 mM CaCl2. Benih terenkapsulasi diletakkan pada media MS dan diberi perlakuan fotoperiodesitas selama 8/16 jam terang/gelap, 10/14 jam terang/gelap, 12/12 jam terang/gelap dan 24/0 jam terang/gelap serta diberi perlakuan kandungan sukrosa yaitu tanpa sukrosa (0%), sukrosa 3% dan sukrosa 4,5%. Parameter penelitian yang digunakan yaitu jumlah benih berkecambah, persentase perkecambahan benih sintetik, perhitungan jumlah daun, tinggi planlet dan jumlah akar
Hasil penelitian didapatkan bahwa kandungan sukrosa pada medium dan fotoperiode berpengaruh terhadap jumlah benih berkecambah, persentase perkecambahan benih, jumlah daun dan tinggi planlet secara optimum pada perlakuan fotoperiode 12/12 jam terang/gelap dan kandungan sukrosa 3%. Namun, kandungan sukrosa pada medium dan fotoperiode tidak berpengaruh terhadap jumlah akar.
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One of the techniques for in vitro propagation of Stevia rebaudiana is using synthetic seed technology. Synthetic seeds are defined as encapsulated explants such as somatic embryos, shoots, shoots, aggregates or other tissues that have the ability to develop into plants in in vitro or ex vitro conditions and can retain their potential after storage. This study aims to determine the effect of sucrose content and photoperiod on the growth of synthetic seeds of Stevia rebaudiana Bertoni. The planting material used was the shoots of Stevia rebaudiana, which were encapsulated using 4% sodium alginate and 75 mM CaCl2. The encapsulated seeds were placed on MS medium and given photoperiodic treatment for 24/0 hours light / dark, 16/8 hours light / dark, 6/18 hours light / dark and 12/12 hours light / dark and were treated with sucrose content i.e. without sucrose (0%), sucrose 3% and sucrose 4,5%. The research parameters used were the number of germinated seeds, the percentage of synthetic seed germination, the calculation of the number of leaves, the height of the plantlets and the number of roots.
Sucrose content in the medium and photoperiod affected the number of germinating seeds, percentage of seed germination, number of leaves and plantlet height optimally in the photoperiod treatment of 12/12 hours of light/dark and sucrose content of 3%. However, the sucrose content in the medium and photoperiod did not affect the number of roots.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Benih sintetik, Fotoperiode, Kandungan sukrosa, Pertumbuhan, Stevia rebaudiana, Growth, Photoperiode, Stevia rebaudiana, Sucrose content , Synthetic seed.
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	May Fiatus Sholihah
Date Deposited:	12 Jul 2021 05:27
Last Modified:	12 Jul 2021 05:27
URI:	http://repository.its.ac.id/id/eprint/84313

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