AKTIVITAS SENYAWA TRISINDOLINA 1 TERHADAP PROTEIN p53R2 DAN p53 PADA SEL KANKER PAYUDARA (T47D) MELALUI DOCKING MOLEKULER

Putri, Vencka Azzahra (2021) AKTIVITAS SENYAWA TRISINDOLINA 1 TERHADAP PROTEIN p53R2 DAN p53 PADA SEL KANKER PAYUDARA (T47D) MELALUI DOCKING MOLEKULER. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Kanker payudara merupakan salah satu jenis kanker yang
dapat menyebabkan kematian dan kanker dengan kasus terbanyak
di dunia. Pengobatan kanker seperti kemoterapi dengan agen
doxorubicin masih memiliki kelemahan karena selain membunuh
sel kanker senyawa tersebut juga mempengaruhi sel-sel normal,
sehingga dibutuhkan penemuan pengobatan baru yang lebih efektif
contohnya adalah senyawa Trisindolina 1. Studi mengenai
senyawa trisindolina 1 dari bahan alam yaitu spons Hyrtios altum
terhadap protein p53R2 dan p53 pada sel kanker payudara (T47D)
melalui melalui metode docking molekuler saat ini masih kurang,
sehingga perlu dilakukan penelitian lebih lanjut sebagai salah satu
tahap awal dalam penemuan obat baru. Adanya aktivitas senyawa
trisindolina 1 akan diuji melalui in silico yaitu docking molekuler
menggunakan software Autodock Vina. Berdasarkan hasil docking
yang telah dilakukan, senyawa Trisindolina 1 memililki nilai
energy bebas Gibbs (∆Gbind) yang lebih negative dibandingkan
senyawa doxorubicin baik pada protein p53 maupun p53R2,
sehingga semakin stabil interaksi yang terbentuk antara asam
amino dari protein reseptor dengan ligan, dan diprediksi
aktivitasnya semakin besar. Selain itu, berdasarkan hasil
visualisasi asam amino, senyawa Trisindolina 1 dapat berikatan
dengan asam amino pada active site protein target yang lebih baik
dibandingkan dengan senyawa control yaitu doxorubicin.
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Breast cancer is one type of cancer that can cause death
and cancer with the highest number in the world. Cancer treatment
with doxorubicin agents still has weaknesses in addition to killing
the compound's cells as well because it affects normal cells with a
rapid proliferation rate, so it is necessary to find a new treatment
that is more effective, for example, Trisindolina 1. Studies on
trisindoline 1 compounds from natural ingredients, namely sponsor
Hyrtios altum There is still a lack of p53R2 and p53 proteins in
breast cancer cells (T47D) through the molecular docking method,
so further research is needed as an early stage in the discovery of
new drugs. The activity of the trisindoline 1 compound will be
tested through silico, namely molecular docking using Autodock
Vina software. Based on the docking results that have been carried
out, the Trisindolina 1 compound has a Gibbs free energy value
(∆Gbind) which is more negative than the doxorubicin compound
in both p53 and p53R2 protein, so that the more stable the
interaction formed between the amino acids of the receptor protein
and ligand, it is predicted that the activity will be greater. In
addition, based on the results of visualization of amino acids,
Trisindolina 1 compound can bind to amino acids on the target
protein of the active site better than the control compound, namely
doxorubicin.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Docking, Kanker, p53R2, Trisindolina 1, T47D, Cancer, Docking, p53R2, Trisindolina 1, T47D
Subjects:	Q Science > QH Biology > QH301 Biology
Divisions:	Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Vencka Azzahra Putri
Date Deposited:	31 Jul 2021 16:12
Last Modified:	31 Jul 2021 16:17
URI:	http://repository.its.ac.id/id/eprint/84652

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