Biodekolorisasi Metil Oranye oleh Bakteri Bacillus subtilis

Zakila, Rikza Ayu (2021) Biodekolorisasi Metil Oranye oleh Bakteri Bacillus subtilis. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Pewarna merupakan komponen pendukung estetika dalam industri tekstil, dimana zat warna yang sering digunakan salah satunya adalah metil oranye (MO). Akan tetapi, limbah pewarna tersebut akan membahayakan ekosistem perairan dan kesehatan manusia jika dibuang langsung ke
badan air. Salah satu upaya penanganan limbah pewarna MO yaitu menggunakan metode biodekolorisasi menggunakan bakteri, dimana metode tersebut dinilai cukup efisien dan ramah lingkungan. Bakteri Bacillus subtilis mempunyai kemampuan dalam mendekolorisasi zat warna. Pada penelitian ini, kondisi optimum bakteri B. subtilis dalam
menghilangkan (biodekolorisasi) MO telah diteliti. Biodekolorisasi MO dilakukan pada media LB dengan variasi konsentrasi akhir 50-400 ppm, waktu inkubasi 0-40 jam, suhu inkubasi 25-40 °C, pH 5-9, kondisi statis dan shaker. Presentase dekolororisasi MO tertinggi oleh B. subtilis pada konsentrasi MO 100 ppm yaitu sebesar 98,36 % selama 40 jam waktu inkubasi dengan suhu 40 °C dan pH 7 pada kondisi statis. Hasil menunjukkan bahwa B. subtilis dengan kondisi optimumnya memiliki kemampuan dalam mendegradasi pewarna MO.
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Dye is a component of aesthetic support in the textile industry, which one of the most commonly used dyes is methyl orange (MO). However, the dye waste will endanger aquatic ecosystems and human health if discharged directly into water bodies. One of the efforts to handle MO dye waste is the biodecolorization method using bacteria, due to consider quite efficient and environmentally friendly. Bacillus subtilis bacterium
has the ability to decolorize dyes. In this study, the optimum conditions for B. subtilis bacterium to remove (biodecolorize) MO were investigated.
MO biodecolorization was carried out on LB media with a final concentration variation of 50-400 ppm, incubation time 0-40 hours, incubation temperature 25-40 °C, pH 5-9, static conditions and shaker. The highest percentage of MO decolorization by B. subtilis at 100 ppm MO concentration was 98.36% for 40 hours of incubation with a temperature of 40 °C and pH 7 under static conditions. The results showed that B. subtilis with its optimum conditions had the ability to degrade MO dyes.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	dekolorisasi, biodegradasi, metil orange, B. subtilis decolorization, biodegradation, methyl orange, B. subtilis
Subjects:	Q Science > QR Microbiology > QR74.8 Bacteria T Technology > TD Environmental technology. Sanitary engineering > TD192.5 Bioremediation
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Rikza Ayu Zakila
Date Deposited:	09 Aug 2021 02:35
Last Modified:	09 Aug 2021 02:35
URI:	http://repository.its.ac.id/id/eprint/85182

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