Produksi Spora Bakteri Karbonatogenik Dan Viabilitasnya Pada Media Pembawa Pasir-Semen

Apriliani, Devinta (2021) Produksi Spora Bakteri Karbonatogenik Dan Viabilitasnya Pada Media Pembawa Pasir-Semen. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Bakteri karbonatogenik merupakan bakteri yang memiliki kemampuan mempresipitasi kalsium karbonat (CaCO3), mampu hidup di lingkungan alkali dan dapat membentuk spora yang tahan terhadap lingkungan ekstrim. Bakteri karbonatogenik dapat memperkuat struktur beton, beberapa spesies yang mampu membentuk spora berpotensi dimanfaatkan sebagai penguat biobeton. Tujuan penelitian adalah mendapatkan biomassa spora yang optimal dan mengetahui viabilitas spora pada media pembawa pasir, semen dan campuran pasir-semen. Isolat yang digunakan adalah Bacillus JA1, JB3, SU1, AK4, Lysinibacillus JB2, dan Sporosracina JA4. Produksi spora dilakukan pada media Yeast Urea (YU) broth dengan perlakuan suhu 70°C selama 20 menit. Spora yang terbentuk disimpan pada media pembawa pasir, semen, dan campuran pasir semen selama 1 bulan. Viabilitas spora divisualisasi menggunakan metode Total Plate Count (TPC). Hasil penelitian menunjukkan semua isolat uji mampu menghasilkan spora pada media YU broth. Bacillus AK4 menghasilkan biomassa kering spora tertinggi yaitu 196 mg/L. Pengamatan viabilitas spora setelah penyimpanan 2 jam, spora mampu tumbuh kembali setelah disimpan dalam media pembawa pasir, semen dan campuran pasir-semen.
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Carbonatogenic bacteria is bacteria group that has ability to precipitate calcium carbonate (CaCO3), endures in an alkaline environments, and forms endospores which resistant to harsh physical condition. Carbonatogenic bacteria is used to strengthen the concrete structure, some of its species formed endospores that have been applied as reinforcement for bio-concrete. The objectives of this study were to obtain optimal spore biomass and determine endospores viability in tested carrier media such as sand, cement and sand-cement mixture. The tested isolates were Bacillus JA1, JB3, SU1, AK4, Lysinibacillus JB2, and Sporosracina JA4. The production of endospores was carried out on Yeast Urea (YU) broth with a temperature treatment of 70°C for 20 minutes. The formed endospores were stored in carrier medium of sand, cement, and a mixture of cement sand for 1 month. Spore viability was conducted using Total Plate Count (TPC) method. The results showed that all isolates were able to produce endospores on YU broth. Bacillus AK4 produced the highest endospores dry biomass, which was 196 mg/L. Observation of endospores viability after 2 hours of storage, endospores were still able to grow after stored in a carrier medium of sand, cement and sand-cement mixture.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Bakteri karbonatogenik, Media YU, Pasir-Semen, Spora, Viabilitas, Carbonatogenic bacteria, YU Media, Sand-Cement, Spore, Viability
Subjects:	Q Science > QR Microbiology > QR74.8 Bacteria
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology
Depositing User:	Devinta Apriliani
Date Deposited:	12 Aug 2021 07:36
Last Modified:	12 Aug 2021 07:36
URI:	http://repository.its.ac.id/id/eprint/85650

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