Resistensi Dan Bioakumulasi Bakteri Karbonatogenik Terhadap Logam Besi (Fe)

Rahmawati, Afianita Dian (2021) Resistensi Dan Bioakumulasi Bakteri Karbonatogenik Terhadap Logam Besi (Fe). Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Abstrak
Bakteri karbonatogenik adalah bakteri yang dapat menghasilkan kalsium karbonat (CaCO3). Di dalam struktur beton terdapat tulangan baja yang merupakan campuran unsur karbon (C) dan besi (Fe). Fe tergolong logam berat yang dalam konsentrasi tinggi bersifat toksik terhadap bakteri. Tujuan penelitian adalah mengetahui resistensi dan bioakumulasi bakteri karbonatogenik terhadap Fe. Isolat yang digunakan adalah Bacillus JA1, JB3, SU1, AK4, Lysinibacillus JB2, dan Sporosarcina JA4. Uji resistensi dan viabilitas dilakukan di Minimal Salt Medium (MSM)-broth yang mengandung Fe 1 ppm, 5 ppm, dan 10 ppm. Uji bioakumulasi menggunakan MSM-broth yang mengandung Fe 5 ppm dan 10 ppm. Uji viabilitas dilakukan dengan menumbuhkan isolat setelah terpapar Fe di medium NA-agar. Konsentrasi Fe yang diakumulasi isolat diukur dengan Atomic Absorption Spectrophotometry (AAS) di Laboratorium Balai Riset dan Standarisasi Industri Surabaya. Hasil dari penelitian adalah semua isolat resisten terhadap logam Fe sampai konsentrasi 10 ppm. Kemampuan bioakumulasi Fe yang terbaik dimiliki oleh Bacillus JA1, yaitu 9,19 ppm pada pemaparan Fe 10 ppm dalam waktu 2 jam dengan efisiensi bioakumulasi 91,85 %.
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Abstract
Carbonatogenic bacteria are able to produce calcium carbonate (CaCO3). Steel reinforcement of concrete is mainly made of the mixture of carbon (C) and ferrous elements. Ferrous is a heavy metal that essential for bacteria growth. However ferrous become toxic when its concentration is too high. The objective of this study was to determine carbonatogenic bacteria resistance and bioaccumulation to ferrous. The isolates used in this study were Bacillus JA1, JB3, SU1, AK4, Lysinibacillus JB2, and Sporosarcina JA4. Resistance and viability tests were carried out on Minimal Salt Medium (MSM)-broth containing ferrous 1 ppm, 5 ppm, and 10 ppm. Bioaccumulation test using MSM-broth containing ferrous 5 ppm and 10 ppm. Bacteria viability determined by growing the isolates after ferrous exposure in NA-agar medium. Accumulated ferrous concentration was measured using Atomic Absorption Spectrophotometry (AAS). All of tested carbonatogenic bacteria are resist to 10 ppm of ferrous. Bacillus JA1 has the highest bioaccumulation ability which was 9,19 ppm after 2 hours exposure of 10 ppm ferrous and its ferrous bioaccumulation efficiency was 91,85%.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Bioakumulasi, Fe, Resistensi, Viabilitas Bioaccumulation, Ferrous, Resistance, Viability
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology
Depositing User:	Afianita Dian Rahmawati
Date Deposited:	14 Aug 2021 03:59
Last Modified:	14 Aug 2021 03:59
URI:	http://repository.its.ac.id/id/eprint/86087

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