Biodegradasi Pewarna Reactive Black 5 Oleh Bakteri Pseudomonas Aeruginosa

Safiranisa, Fatia (2021) Biodegradasi Pewarna Reactive Black 5 Oleh Bakteri Pseudomonas Aeruginosa. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Limbah industri tekstil merupakan salah satu sumber polutan yang dapat mencemari lingkungan dan menimbulkan berbagai masalah. Salah satu jenis limbah yang dihasilkan dari industri tekstil adalah limbah pewarna reactive black 5 (RB5). Penghilangan warna RB5 efektif dapat berlangsung secara biologis yaitu dengan menggunakan mikroorganisme. Pada penelitian kali ini dlakukan biodegradasi pewarna RB5 dengan bakteri Pseudomonas aeruginosa dengan kondisi optimumnya yang dianalisis dengan instrumen UV-Vis. Biodegradasi dilakukan dengan pada media cair Luria-Bertani broth (LB) dengan konsentrasi 50 mg/L, waktu inkubasi 48 jam, suhu 40 ℃, pH 7, dan dalam kondisi didiamkan. Berdasarkan penelitian ini terbukti bahwa bakteri P. aeruginosa mampu mendegradasi RB5 sebesar 91%.
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Textile industry wastes are kind of pollutans that can pollute the environment and can be serious problems. One type of waste generated from the textile industry is Reactive black 5 (RB5). Effective decolorization of RB5 can be carried out biologically using microorganisms. In this study, RB5 was removed by biodegradation process using the bacterium Pseudomonas aeruginosa with optimum condition and was analyzed using UV-Vis instrument. Degradation was perfomed on Luria-Bertani medium (LB) with concentration of RB5 50 mg/L with an incubation time of 48 hours, at temperature 40 ℃, oH 7, and static conditions. As a result, it was proven that P. aeruginosa was able to degrade RB5 approximately 91%.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Biodegradasi, Pseudomonas aeruginosa, reactive black 5
Subjects:	Q Science
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Fatia Safiranisa
Date Deposited:	14 Aug 2021 09:21
Last Modified:	14 Aug 2021 09:21
URI:	http://repository.its.ac.id/id/eprint/86631

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