Studi In Silico Senyawa (6)-GINGEROL Jahe Merah (Zingiber officinale var. Rubrum) Terhadap Protein CpsB Pada Streptococcus pneumoniae

Maufiroh, Sitti Aisyatul (2021) Studi In Silico Senyawa (6)-GINGEROL Jahe Merah (Zingiber officinale var. Rubrum) Terhadap Protein CpsB Pada Streptococcus pneumoniae. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Pneumonia merupakan salah satu penyakit infeksi saluran pernafasan yang menjadi penyebab utama kematian di seluruh dunia. Patogen umum penyebab pneumonia yaitu bakteri Streptococcus pneumoniae. Faktor virulensi utama pada bakteri ini terletak pada kapsul polisakarida (CPS) yang berperan dalam menggganggu mekanisme fagositosis. Penghambatan regulasi biosintesis kapsul polisakarida diketahui dapat menurunkan tingkat produksi kapsul pada
S. pneumonia melalui protein target CpsB (protein tirosin fosfatase). Salah satu tanaman herbal yang berpotensi sebagai antibakteri yaitu jahe merah (Zingiber officinale var. Rubrum yang mensistesis senyawa metabolit (6) gingerol. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa (6)gingerol terhadap kapsul polisakarida S. Pneumonia. Metode yang digunakan adalah docking molekuler ligan dan reseptor menggunakan Pyrx terhadap protein target CpsB, dengan kontrol positif obat Ceftriaxone. Nilai docking ditunjukan dengan binding affinity (ΔG paling negatif) yang menggambarkan energi total ikatan ligan dan reseptor. Hasil docking didapatkan bahwa (6)gingerol dan Ceftriaxone berpotensi sebagai inhibitor protein CpsB dengan nilai binding affinity sebesar -5.8 Kkal/mol dan -8.6 Kkal/mol. Jenis asam amino yang terlibat dalam interaksi liganreseptor yaitu Arginin, Prolin, Serin, dan Histidin.
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Pneumonia is a respiratory infection which is the leading cause of death worldwide. The common pathogen that causes pneumonia is Streptococcus pneumoniae. The main virulence factor in these bacteria lies in the polysaccharide capsule (CPS) which plays a role in disrupting the phagocytosis mechanism. Inhibition of biosynthetic regulation of CPS is known to reduce the level of capsule production in S. pneumonia through the target protein CpsB (Tyrosine protein phosphatase). Research on CPS is still being developed today. One of the herbal plants has the potential to be antibacterial is the red ginger (Zingiber officinale var. Rubrum) which synthesizes the compound (6)–gingerol. This study aims to determine the activity of (6)-gingerol compound against polysaccharide capsules of S. pneumoniae. The method used is molecular docking of ligands and receptors using Pyrx against the target protein CpsB, with a positive control of Ceftriaxone. The docking value is indicated by binding affinity (the most negative ΔG) which describes the total binding energy of the ligand and receptor. The docking results showed that (6)-gingerol and Ceftriaxone were potential inhibitors of CpsB protein with binding affinity values of -5.8 Kcal/mol and -8.6 Kcal/mol. The types of amino acids involved in the ligand-receptor interaction are Arginine, Proline, Serine, and Histidine

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Antibacterial, Docking, Polysaccharide Capsule, Virulance, Antibakteri, Docking, Kapsul Polisakarida, Virulensi
Subjects:	Q Science > QH Biology > QH301 Biology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Sitti Aisyatul Maufiroh
Date Deposited:	25 Aug 2021 02:56
Last Modified:	25 Aug 2021 02:56
URI:	http://repository.its.ac.id/id/eprint/89346

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