Rosyidah, Hafildatur (2021) Metode untuk Membedakan Bakteri Staphylococcus Aureus dan Escherichia Coli Berbasis Spektrofotometri Fluoresens. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Penelitian mengenai metode analisis untuk membedakan bakteri S. aureus dan E. coli telah dilakukan menggunakan spektrofotometri fluoresens. Spektrofotometri fluoresens merupakan metode analisis berdasarkan emisi suatu zat yang telah menyerap cahaya atau radiasi elektromagnetik lainnya. Pada penelitian ini, regenerasi dan kultur bakteri dilakukan menggunakan media padat Nutrien Agar (NA) dan larutan blanko Phosphate Buffer Saline (PBS) untuk pengukuran dengan fluoresens. Berdasarkan spektra fluoresens diperoleh nilai panjang gelombang emisi dan eksitasi maksimum untuk membedakan kedua jenis bakteri. Bakteri S. aureus memiliki puncak emisi maksimum pada panjang gelombang 441nm dan 570nm dan eksitasi pada 218nm dengan jumlah koloni yang terbentuk 2,4×107CFU/mL. Sedangkan bakteri E. coli memiliki puncak emisi maksimum pada panjang gelombang 451nm dan 586nm serta puncak eksitasi pada 216nm dengan jumlah koloni yang terbentuk 1,7×107CFU/mL. Dari hasil tersebut menunjukkan bahwa metode spektrofotometri fluoresens dapat digunakan sebagai metode alternatif dalam membedakan jenis bakteri patogen secara cepat, sensitif, dan praktis.
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Research on analytical methods to differentiate S. aureus and E. coli bacteria has been carried out using fluorescence spectrophotometry. Fluorescent spectrophotometry is an analytical method based on the emission by a substance that has absorbed light or other electromagnetic radiation. In this study, bacterial regeneration and culture were carried out using Nutrient Agar (NA) and Phosphate Buffer Saline (PBS) blank solution for fluorescence measurements. Based on the fluorescence spectra, the maximum emission and excitation wavelength values were obtained to differentiate the two types of bacteria. S. aureus have maximum emission peaks at wavelengths of 441nm and 571nm and excitation of 218nm with the number of colonies formed 2.4×107CFU/mL. Meanwhile, E. coli have maximum emission peaks at wavelengths of 451nm and 586nm and excitation peaks at 216nm with the number of colonies formed 1.7×107CFU/mL. These results indicate that the fluorescence spectrophotometry method can be used as an alternative method to rapidly, sensitively, and practically distinguish the types of pathogenic bacteria.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Uncontrolled Keywords: | S. aureus, E. coli, Spektrofotometri Fluoresens, Eksitasi, Emisi, Fluorescent Spectrophotometry, Excitation, Emission |
| Subjects: | Q Science > QD Chemistry > QD117.S64 Spectrophotometry |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis |
| Depositing User: | Hafildatur Rosyidah |
| Date Deposited: | 28 Aug 2021 00:01 |
| Last Modified: | 28 Aug 2021 00:01 |
| URI: | http://repository.its.ac.id/id/eprint/90229 |
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