Ziyana, Rachma Anni (2022) Penghilangan Warna Metilen Biru Oleh Jamur Filamen Trichoderma Viride Yang Terimobilisasi Pada Matriks SA/PVA/Bentonit. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Metilen biru (MB) merupakan salah satu pewarna yang digunakan dalam industri teksil, akan tetapi dapat menimbulkan bahaya bagi lingkungan dan manusia sehingga diperlukan cara untuk menghilangkannya. Penelitian ini membahas tentang biodegradasi MB oleh jamur filamen Trichoderma viride yang terimobilisasi pada matriks SA/PVA/Bentonit. Penelitian ini dilakukan dengan metode imobilisasi teknik entrapment yang menggabungkan komposisi matriks SA/PVA/Bentonit dengan perbandingan SA:PVA:Bentonit = 2:4:1%. Proses biodekolorisasi dilakukan dengan dua metode yakni free cell dan imobilisasi. Hasil penelitian ini menujukkan pada metode imobilisasi dihasilkan persen dekolorisasi yang lebih maksimal yakni sebesar 95,72
% sedangkan free cell sebesar 55,36 % dengan masa inkubasi selama 7 hari dalam kondisi statis. Keberhasilan imobilisasi dapat dibuktikan dengan spektra FTIR dan visualisasi SEM. Pada uji pengukuran berulang (uji Reusability) Beads imobilisasi menujukkan dapat digunakan kembali selama empat kali siklus. Hasil ini menunjukkan bahwa T. viride yang terimobilisasi pada matriks SA/PVA/Bentonit dapat mendokolorisasi MB dan dapat dijadikan alternatif untuk mengurangi permasalahan dari limbah pencemar yang ada.
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Methylene blue (MB) is one of the dyes used in the textile industry, but it can pose a danger to the environment and humans, so a method is needed to remove it. This study discussed biodegradation of MB by immobilized filamentous fungus Trichoderma viride into SA/PVA/bentonite matrix. In this study, the immobilization method of entrapment technique was combined the composition of the matrix SA/PVA/Bentonite with a ratio 2:4:1 %. The biodecolorization process was carried out by two methods, namely free cell and immobilization. The results of this study showed that the immobilization method produced a maximum decolorization percentage of 95.72% meanwhile free cell 55.36 % with an incubation period of 7 days under static conditions. In this study the success of immobilization can be proven by FTIR spectra and SEM visualization. In the repeated measurement test (Reusability test), the immobilized beads showed that they could be reused for four cycles. These results indicated that T. viride immobilized on the SA/PVA/Bentonite matrix can colorize MB and can be used as an alternative to reduce the problem of existing pollutant waste.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Uncontrolled Keywords: | Biodecolorization, Methylene Blue, Trichoderma viride, Immobilization, Bentonite, Biodekolorisasi, Metilen Biru, Imobilisasi, Bentonit, |
| Subjects: | Q Science Q Science > QD Chemistry Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis |
| Depositing User: | Rachma Anni Ziyana |
| Date Deposited: | 07 Feb 2022 02:19 |
| Last Modified: | 17 Dec 2024 03:20 |
| URI: | http://repository.its.ac.id/id/eprint/92895 |
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