Dusabe, Simpliste (2022) Production Of Bioelectricity From Food Waste And Sidoarjo Mud Using Single Chamber Microbial Fuel Cells With The Help Of Fungi Rich In Hydrolytic Enzymes For A Sustainable Future. Masters thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Rapid global economic growth will lead to a considerable increase in food waste (FW) production. Improper food waste management is still a significant challenge that the entire world faces as it contaminates the environment and endangers human and animal health. However, FW can contribute significantly to creating a safe environment and renewable energy if processed. Previous researchers focused on removing organic pollutants and have omitted energy extraction from them. However, Microbial fuel cell technology can produce electricity through pollutant degradation by the action of microorganisms. Food waste was first blended and diluted at different concentrations in this research. After a simple solid-liquid separation, the liquid was hydrolysed by fungi rich in hydrolytic enzymes at their log phases. The hydrolysate was mixed with Sidoarjo mud and fed to the Single Chamber microbial fuel cell (SC-MFC) for electricity production. Shewanella oneidensis MR-1 was used in this research as it is one of the widely used exoelectrogen in MFC with high productivity. In addition, Sidoarjo mud was mixed with food waste as it contains microorganisms that might play a key role in MFC electron transfer. This research aims to produce electricity while reducing the organic matter from food waste by integrating the hydrolytic microorganism and microbial fuel cells. Moreover, the ability of microorganisms to hydrolyse FW towards glucose production, the kinetic study related to the organic compound removal rate in this work and the effect of Sidoarjo mud on the growth of bacteria in Shewanella oneidensis MR1 were discussed. Experiments revealed that the highest glucose obtained was 11.36 g/L by mixing all the fungi with a concentration ratio of (ᴡ/ν) 2:1. The highest total power density of 53.311 W/m2, 78.38% of Biological Oxygen Demand (BOD5) removal and 84.87% of Chemical Oxygen Demand (COD) removal were also obtained by mixing all the fungi with a concentration ratio (ᴡ/ν) of 2:1 within 18th days of MFC operation. The maximum specific growth rate (ko) and the saturation constant (Km) were 0.07789 Day -1 and 3015.48 mg/L, respectful. The results showed that Sidoarjo mud doesn’t inhibit the growth of Shewanella oneidensis MR1 in MFC operation. Therefore, the current findings indicate that hydrolytic microorganisms could greatly enhance food waste degradation and become an MFC carbon source for electricity production.
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Pertumbuhan ekonomi global yang cepat akan menyebabkan peningkatan yang cukup besar dalam produksi limbah makanan (FW). Pengelolaan limbah makanan yang tidak tepat menjadi tantangan signifikan bagi seluruh dunia karena mencemari lingkungan dan membahayakan kesehatan manusia dan hewan. Jika diproses, FW dapat berkontribusi secara signifikan untuk menciptakan lingkungan yang aman dan energi terbarukan. Peneliti sebelumnya berfokus pada menghilangkan polutan organik dan menghilangkan ekstraksi energi. Namun, teknologi Microbial fuel cell dapat menghasilkan listrik melalui degradasi polutan oleh mikroorganisme. Dalam penelitian ini, FW diblender dan diencerkan terlebih dahulu dengan konsentrasi yang berbeda. Setelah pemisahan padat-cair yang sederhana, cairan tersebut dihidrolisis oleh jamur yang kaya akan enzim hidrolitik pada fase lognya. Hidrolisat dicampur dengan lumpur Sidoarjo dan diumpankan ke Single Chamber microbial fuel cells (SC-MFC) untuk produksi listrik. Shewanella oneidensis MR-1 digunakan dalam penelitian ini karena merupakan salah satu exoelectrogen yang banyak digunakan di MFC dengan produktivitas tinggi. Selain itu, lumpur Sidoarjo bercampur dengan FW karena mengandung mikroorganisme yang mungkin berperan penting dalam transfer electron di MFC. Penelitian ini bertujuan untuk menghasilkan listrik sekaligus mengurangi bahan organik dari FW dengan mengintegrasikan mikroorganisme hidrolitik dan microbial fuel cell. Selain itu, kemampuan mikroorganisme untuk menghidrolisis FW terhadap produksi glukosa , studi kinetik terkait dengan laju penyisihan senyawa organik dalam pekerjaan ini dan pengaruh lumpur Sidoarjo terhadap pertumbuhan bakteri di Shewanella oneidensis MR1 dibahas . Hasil menunjukkan bahwa glukosa tertinggi yang diperoleh 11,36 g/L dengan mencampur semua jamur dengan rasio konsentrasi (ᴡ/ν) 2:1. Total Power density tertinggi 53,311 W/m2, Biological Oxygen Demand (BOD5) removal 78,38% dan Chemical Oxygen Demand (COD) removal 84,87% juga diperoleh dengan mencampurkan semua jamur dengan rasio konsentrasi (ᴡ/ν) 2:1 dalam waktu 18 hari sejak MFC beroperasi. Maximum specific growth rate (ko) dan saturation constant (Km) adalah 0,07789 Hari-1 dan 3015,48 mg/L. Hasil penelitian menunjukkan bahwa lumpur Sidoarjo tidak menghambat pertumbuhan Shewanella oneidensis MR1 pada operasi MFC. Oleh karena itu, temuan saat ini menunjukkan bahwa mikroorganisme hidrolitik dapat sangat meningkatkan degradasi limbah makanan dan menjadi sumber karbon MFC untuk produksi listrik.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Food waste, Microbial fuel cell, Hydrolytic microorganisms, Shewanella oneidensis MR-1, Sidoarjo Mud, Bioelectricity, Limbah makanan, Mikroorganisme Hidrolitik, Lumpur Sidoarjo, Biolistrik |
| Subjects: | Q Science > QD Chemistry T Technology > TP Chemical technology T Technology > TP Chemical technology > TP255 Electrochemistry, Industrial. |
| Divisions: | Faculty of Industrial Technology and Systems Engineering (INDSYS) > Chemical Engineering > 24101-(S2) Master Thesis |
| Depositing User: | Simpliste Dusabe |
| Date Deposited: | 25 May 2022 03:26 |
| Last Modified: | 31 Oct 2022 02:48 |
| URI: | http://repository.its.ac.id/id/eprint/94894 |
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