Biodegradasi Metilen Biru Menggunakan Kultur Campuran Jamur Filamen dan Bakteri Bacillus subtilis yang Terimobilisasi pada Matriks SA-PVA-Bentonit

Rohmah, Alya Awinatul (2022) Biodegradasi Metilen Biru Menggunakan Kultur Campuran Jamur Filamen dan Bakteri Bacillus subtilis yang Terimobilisasi pada Matriks SA-PVA-Bentonit. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Limbah metilen biru (MB) merupakan salah satu limbah pewarna tekstil yang bersifat toksik dan sukar dihilangkan. Bioremediasi dengan menggunakan mikroorganisme telah terbukti dapat mengurangi polutan yang berbahaya di
lingkungan. Di sisi lain, teknik imobilisasi mempunyai keuntungan yang lebih efektif dibandingkan dengan sel bebas. Oleh karena itu, tujuan penelitian ini adalah
menyelidiki kemampuan kultur campuran Trichoderma viride-Bacillus subtilis (TV-BS) dan Aspergillus oryzae-Bacillus subtilis (AO-BS) yang diimobilisasi pada matriks SA-PVA-Bentonit untuk mendegradasi dan mendekolorisasi MB. Hasil
penelitian menunjukkan bahwa pada kondisi statis dengan massa bead 2,5 g dalam waktu 120 menit, bead kosongan; bead TV-BS; dan bead AO-BS dapat mendekolorisasi 50 mg/L MB sebesar 79,40; 96,55; dan 95,29%. Pada kondisi
pengadukan, bead TV-BS dan bead AO-BS memiliki hasil persen dekolorisasi MB yang lebih tinggi yaitu 97,87 dan 97,01%. Produk metabolit hasil degradasi dianalisis dengan menggunakan LC-MS dan diprediksi metabolit sekundernya
sebagai 4-amino-2-sulfobenzenaminium, tionin, dan 3-((3-(dimetilamino) fenil)sulfinil)-N1,N1-dimetilbenzena-1,4-diamin. Struktur bead imobilisasi dikarakterisasi dengan menggunakan instrument SEM, FTIR, XRD, dan uji adsorpsi desorpsi nitrogen. Kinetika adsorpsi bead hasil sintesis, mengikuti kinetika orde dua semu dan isotermal adsorpsinya mengikuti isotermal Langmuir. Hasil uji aktivitas enzim lakase membuktikan bahwa terdapat enzim lakase yang berperan
pada proses dekolorisasi MB. Berdasarkan semua hasil uji dan analisa yang telah dilakukan, dapat dibuktikan bahwa bead kultur campuran mampu mendegradasi MB sebesar 12,98% (TV-BS) dan 11.06% (AO-BS) pada suhu 30 °C, waktu 24 jam dan keadaan statis.
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Methylene blue (MB) waste is a textile dye waste which is toxic and difficult to remove. Bioremediation using microorganisms has been shown to reduce harmful pollutants in the environment. On the other hand, the immobilization technique is more effective than free cells. Therefore, the aim of this study was to investigate the ability of mixed cultures of Trichoderma viride�Bacillus subtilis (TV-BS) and Aspergillus oryzae-Bacillus subtilis (AO-BS) immobilized into SA-PVA-Bentonite matrix were used to degrade and decolorize MB. The results showed that under static conditions by using 2.5 g mass of bead within 120 minutes, the empty bead; TV-BS bead; and AO-BS bead could
decolorize 50 mg/L MB up to 79.40; 96.55; and 95.29%. Under stirring conditions, TV-BS bead and AO-BS bead had higher percentage of MB decolorization, approximately 97.87 and 97.01%, respectively. The metabolite degradation
products were analyzed using LC-MS and predicted as 4-amino-2-sulfobenzenaminium, thionine, and 3-((3-(dimethylamino) phenyl)sulfinyl)-N1,N1-dimethylbenzene-1,4-diamine. The immobilized bead structure was characterized
using SEM, FTIR, XRD instruments, and nitrogen desorption adsorption test. The adsorption kinetics of the synthesized bead followed pseudo second order kinetic and the adsorption isothermal followed the Langmuir isothermal. The results of the laccase enzyme activity test proved that there was a laccase enzyme activity, which played a role in the MB decolorization process. Based on all the results, it can be
proven that mixed culture beads are able to degrade MB until 12.98% (TV-BS) and 11.06% (AO-BS) at 30 °C for 24 hours under static condition.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	Biodegradation, Biodecolorization, Methylene Blue, Bacillus subtilis, Trichoderma viride, Aspergillus oryzae, immobilization, Biodegradasi, Biodekolorisasi, Metilen Biru
Subjects:	Q Science > QP Physiology > QP517.B5 Biodegradation.
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47101-(S2) Master Thesis
Depositing User:	- Davi Wah
Date Deposited:	02 Dec 2022 02:25
Last Modified:	02 Dec 2022 02:25
URI:	http://repository.its.ac.id/id/eprint/95128

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