Sintesis Hidrotermal Carbon Nanorod Terdoping Nitrogen Sebagai Material Antibakteri

Muliyono, Charisa Virga (2022) Sintesis Hidrotermal Carbon Nanorod Terdoping Nitrogen Sebagai Material Antibakteri. Other thesis, Institut Teknologi Sepuluh Nopember Surabaya.

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Abstract

Material nano dalam bentuk alotrof karbon dapat menunjukkan aktivitas bakterisidal dan bakteriostatik yang sangat baik terhadap berbagai mikroorganisme sehingga material nano dapat dimanfaatkan sebagai material antibakteri. Carbon nanorod (CNRs) adalah salah satu material nano alotrof karbon yang dapat dimanfaatkan sebagai material antibakteri. Penambahan doping nitrogen seperti pada alotrof karbon lainnya (grafena, carbon dots, carbon nanotube) diharapkan dapat meningkatkan aktivitas antibakterinya. Pada penelitian ini, dikembangkan sintesis secara hidrotermal carbon nanorod terdoping nitrogen (N-CNRs) dan analisis karakteristik N-CNRs dikorelasikan terhadap aktivitas antibakterinya. N-CNRs disintesis dengan variasi sumber doping nitrogen (trietilamin, urea, dan amonia) dengan perbandingan massa karbon terhadap nitrogen C:N (25:1; 50:1; dan 200:1). Karakterisasi dilakukan menggunakan spektroskopi Ultraviolet-Visible (UV-Vis), dan Fourier Transform Infrared (FTIR) serta Scanning Electron Microscope – Energy Dispersive X-ray (SEM-EDX), dan Dynamic Ligth Scattering (DLS). Aktivitas antibakteri diuji dalam kondisi gelap dan terang dengan metode mikrodilusi dan Kirby Bauer untuk menentukan minimum inhibitory concentration (MIC) terhadap bakteri E. coli (Gram negatif) dan S. aureus (Gram positif). Hasilnya, ukuran partikel terkecil diperoleh dari N-CNRs dengan sumber doping nitrogen berupa urea, serta muatan permukaan N-CNRs yang bernilai negatif. N-CNRs yang dihasilkan memiliki MIC terbaik sebesar 0.125 mg/ml. Hasil penelitian ini juga menunjukkan bahwa ukuran N-CNRs dan kondisi terang membantu meningkatkan aktivitas antibakteri akibat kombinasi interaksi permukaan dan Reactive Oxygen Species (ROS)
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Nanomaterials in the form of carbon allotropes might exhibit excellent bactericidal and bacteriostatic activity against various microorganisms and hence, nanomaterials can be used as antibacterial agents. Carbon nanorod (CNRs) is one of carbon allotropes that can be employed as antibacterial agents. Nitrogen doping as also found in other carbon allotropes (graphene, carbon dots, carbon nanotubes) is expected to increase its antibacterial activity. In this study, hydrothermal synthesis of nitrogen-doped carbon nanorods (N-CNRs) was developed and the physicochemical characteristics of N-CNRs are correlated to their antibacterial activity. N-CNRs was synthesized using various nitrogen doping sources, i.e., triethylamine, urea, and ammonia, with a carbon to nitrogen (C:N) ratio of 25:1; 50:1; and 200:1. Characterization was carried out using Ultraviolet-Visible (UV-Vis), dan Fourier Transform Infrared (FTIR) serta Scanning Electron Microscope – Energy Dispersive X-ray (SEM-EDX), dan Dynamic Ligth Scattering (DLS). Antibacterial activity was tested under dark and light conditions using microdilution and Kirby Bauer methods to determine the minimum inhibitory concentration (MIC) for E. coli (Gram negative) and S. aureus (Gram positive). As a result, the smallest particle size was obtained from N-CNRs with urea nitrogen doping as a source, and the surface charge of N-CNRs was negative. N-CNRs had the best MIC of 0.125 mg/ml. In addition, the results indicate that the size of N-CNRs and light condition play role in enhancing the antibacterial activity due to combined effect of surface interaction and reactive oxygen species (ROS)

Item Type:	Thesis (Other)
Additional Information:	RSF 620.189 34 Mul s-1
Uncontrolled Keywords:	Antibakteri, Material nano, Alotrof karbon, MIC
Subjects:	T Technology > TP Chemical technology > TP248 Nanogels. Nanoparticles.
Divisions:	Faculty of Industrial Technology and Systems Engineering (INDSYS) > Physics Engineering > 30201-(S1) Undergraduate Thesis
Depositing User:	EKO BUDI RAHARJO
Date Deposited:	10 Jan 2023 01:59
Last Modified:	10 Jan 2023 01:59
URI:	http://repository.its.ac.id/id/eprint/95351

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