Analisis Bioreduksi Besi(III) Menjadi Besi(II) oleh Bakteri Isolat Lahar Semeru

Zahranisa, Kamila (2023) Analisis Bioreduksi Besi(III) Menjadi Besi(II) oleh Bakteri Isolat Lahar Semeru. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Sampel isolat bakteri yang diambil dari lahar Gunung Semeru dengan koordinat LU: 90o96’126.959” BT: 72o08’12.318” dianalisis dalam mereduksi Fe(III) menjadi Fe(II) dengan melakukan regenerasi menggunakan metode gores 4 kuadran, pembuatan suspensi bakteri yang kemudian direaksikan dengan larutan Fe(III), serta penentuan kadar persen rekoveri besi menggunakan metode spektrofotometer UV-Vis. Penelitian ini bertujuan untuk mengetahui potensi isolat bakteri dalam mereduksi Fe(III) menjadi Fe(II),serta bermanfaat dalam pengembangan teknologi pengendalian pencemaran lingkungan akibat logam berat B3 oleh aktivitas industri. Variabel yang ditentukan adalah kondisi sel bakteri yaitu sel bakteri hidup dan sel bakteri mati serta spesies bakteri, antara lain Pseudomonas sp., Bacterium strain BS2049, dan Uncultured bacterium clone 1. Hasil yang didapat dari penelitian ini adalah persen rekoveri kadar besi dan waktu yang dibutuhkan untuk mereduksi besi oleh masing-masing bakteri. Sel bakteri Pseudomonas sp.hidup berhasil mereduksi besi dengan waktu inkubasi selama 1 jam dan mendapatkan persen rekoveri sebesar 94,05%, sedangkan sel bakteri Pseudomonas sp. mati berhasil mereduksi besi dengan waktu inkubasi selama 6 jam dan mendapatkan persen rekoveri sebesar 75,73%. Selain itu, varian bakteri lain yaitu sel Bacterium strain BS2049 hidup memerlukan waktu 4 jam untuk mereduksi besi dan mendapatkan nilai persen rekoveri sebesar 93,58% sedangkan sel Bacterium strain BS2049 mati mendapatkan persen rekoveri sebesar 53,61% setelah mereduksi besi selama 6 jam. Sel bakteri Uncultured bacterium clone 1 hidup berhasil mereduksi besi dengan waktu inkubasi selama 96 jam dan mendapatkan persen rekoveri sebesar 101,26%, sedangkan sel bakteri Uncultured bacterium clone 1 mati tidak menunjukkan kemampuan untuk mereduksi besi yang ditunjukkan dengan nilai persen rekoveri sebesar 0%
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Bacterial isolates collected from the Mount Semeru lava with coordinates of LU: 90o96’126.959” BT: 72o08’12.318” were analyzed to obtain reduction potential of Fe(III) to Fe(II) by regenerating the bacteria using the 4 quadrant streak method, preparing liquid cultures which were then reacted with Fe(III) solution, and determining the percentage of iron recovery using UV-Vis spectrophotometer instrument. This study aims to determine the potential of bacterial isolates in reducing Fe(III) to Fe(II) in order to develop technology to control environmental pollution due to heavy metals by industrial activities. Variables that were varied were the condition of bacterial cells, namely living and dead bacterial cells, as well as bacterial species such as Pseudomonas sp., Bacterium strain BS2049, and Uncultured bacterium clone 1. The results obtained from this study were the percent of iron recovery and the time required to reduce iron by each bacterium. Living Pseudomonas sp. bacteria cells were capable to reduce iron with an incubation time of 1 hour and getting a percent recovery of 94,05%, while the dead bacterial cells of Pseudomonas sp. managed to reduce iron with an incubation time of 6 hours and get a percent recovery of 75,73%. In addition, other bacterial variants, namely living Bacterium strain BS2049 cells required 4 hours to reduce iron and obtained a percent recovery value of 93,58% whereas dead Bacterium strain BS2049 cells obtained a percent recovery of 53,61% after reducing iron for 4 hours. Living Uncultured bacterium clone 1 bacterial cells were capable to reduce iron with an incubation time of 96 hours and obtained a percent recovery of 101,26%, whilst dead Uncultured bacterium clone 1 bacterial cells did not show any ability to reduce iron, which was indicated by the percent recovery value of 0%.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Biorduksi, Besi, Spektrofotometer UV-Vis, Pseudomonas sp., Bacterium strain BS2049, Uncultured bacterium, Persen rekoveri
Subjects:	Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Kamila Zahranisa
Date Deposited:	24 Jan 2023 06:02
Last Modified:	24 Jan 2023 06:02
URI:	http://repository.its.ac.id/id/eprint/95583

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