Investigasi Pengaruh Suhu Inkubasi Kultur Bakteri Terhadap Infeksi Intranasal Staphylococcus aureus Pada Hewan Model Mus musculus

Ashari, Bethari Auchenfloretta Zahra (2023) Investigasi Pengaruh Suhu Inkubasi Kultur Bakteri Terhadap Infeksi Intranasal Staphylococcus aureus Pada Hewan Model Mus musculus. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Staphylococcus aureus umumnya dapat ditemukan secara komensal di berbagai lokasi pada tubuh diantaranya yaitu kulit, vagina, organ pencernaan, ketiak, dan hidung sebagai akses utama transmisi bakteri ke organ dalam. Rongga hidung dapat dikatakan sebagai akses utama infeksi bakteri S. aureus diakibatkan adanya rongga terbuka untuk masuk ke dalam tubuh. Adapun strain S. aureus yang saat ini masih menjadi ancaman di dunia medis yaitu strain yang memiliki resistensi terhadap antibiotik methicillin atau dikenal sebagai Methicillin Resistant S. aureus (MRSA). Di Indonesia diketahui prevalensi infeksi akibat MRSA diketahui sekitar 25 – 65% dengan rata-rata nasional sebesar 38%. Individu dengan infeksi MRSA biasanya mengalami inflamasi sistemik akibat proinflamasi sitokin oleh sel imun. Pertumbuhan bakteri pada umumnya bergantung pada temperatur dan pH lingkungan. Temperatur diketahui menjadi faktor utama pertumbuhan optimum MRSA dan produksi pirogen Staphylococcal Enterotoxin A (SEA) pada media kultur. SEA diproduksi pada temperature sekitar 10–46 °C, dengan temperatur optimum yaitu 37–45 °C. Investigasi lebih lanjut mengenai pengaruh t¬¬¬¬¬¬emperatur pada infeksi bakteri MRSA akan dilakukan penelitian secara in-vivo menggunakan hewan model Mus musculus DDY betina usia 6–8 minggu dengan strain MRSA yang ditumbuhkan dan diinkubasi pada temperatur 37 °C dan 39 °C. Berdasarkan penelitian ini diketahui bahwa tidak ada perbedaan signifikan mengenai pengaruh inkubasi 37 °C dan 39 °C terhadap jumlah kolonisasi bakteri pada organ. Uji survival rate hewan model dilakukan untuk meninjau lebih lanjut pengaruh temperatur terhadap patogenisitas S. aureus USA300. Hasil uji survival rate pada temperatur 39 °C didapatkan penurunan nilai lebih tinggi sebesar 25% dibandingkan dengan temperatur 37 °C sebesar 15%.
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Staphylococcus aureus can generally be found commensal in various locations on the body including the skin, vagina, digestive organs, armpits, and nose as the main access for bacterial transmission to internal organs. The nasal cavity can be said to be the main access for S. aureus bacterial infection due to an open cavity to enter the body. The strain of S. aureus that is currently still a threat in the medical world is a strain that has resistance to the antibiotic methicillin or known as Methicillin Resistant S. aureus (MRSA). In Indonesia, it is known that the prevalence of infection due to MRSA is known to be around 25–65% with a national average of 38%. Individuals with MRSA infection usually experience systemic inflammation due to proinflammatory cytokines by immune cells. Bacterial growth generally depends on the temperature and pH of the environment. Temperature is known to be the main factor for optimum growth of MRSA and pyrogen production of Staphylococcal Enterotoxin A (SEA) in culture media. SEA is produced at temperatures around 10–46°C, with an optimum temperature of 37–45°C. Further investigation of the effect of temperature on MRSA bacterial infection will be carried out in vivo using an animal model of female Mus musculus DDY aged 6–8 weeks with MRSA strain grown and incubated at 37°C and 39°C. Based on this study it is known that there is no significant difference regarding the effect of incubation of 37 °C and 39 °C on the amount of bacterial colonization in organs. Survival rate test of animal models was carried out to further review the effect of temperature on the pathogenicity of S. aureus USA300. The survival rate test results at a temperature of 39 °C showed a higher reduction of 25% compared to a temperature of 37 °C of 15%.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Demam, Infeksi, Intranasal, MRSA, Temperatur, Fever, Infection, Intranasal, MRSA, Temperature
Subjects:	Q Science > QR Microbiology > QR74.8 Bacteria
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Bethari Auchenfloretta Zahra Ashari
Date Deposited:	12 Feb 2023 12:44
Last Modified:	12 Feb 2023 12:44
URI:	http://repository.its.ac.id/id/eprint/96921

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