Potensi Isolat Bakteri dari Lahar Gunung Semeru dalam Bioreduksi Besi(III) menjadi Besi(II) dengan Pengaruh Varian Temperatur

Sinaga, Ivana Caroline (2023) Potensi Isolat Bakteri dari Lahar Gunung Semeru dalam Bioreduksi Besi(III) menjadi Besi(II) dengan Pengaruh Varian Temperatur. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Besi merupakan salah satu unsur dengan kelimpahan terbanyak keempat pada kerak bumi. Kelimpahan besi ini dapat dimanfaatkan oleh makhluk hidup dalam proses metabolismenya. Namun dalam jumlah yang berlebih, besi dapat menimbulkan efek toksik. Perkembangan industri, mendorong peningkatan jumlah industri elektroplating yang menghasilkan berbagai limbah logam berat. Salah satunya yaitu besi. Oleh sebab itu dibutuhkan metode remediasi lingkungan untuk menjaga kelimpahan besi di alam agar tidak membahayakan makhluk hidup dan ekosistem sekitar. Salah satu metode remediasi yang ramah lingkungan dan aman untuk diterapkan yaitu bioremediasi dengan memanfaatkan aktivitas mikroorganisme. Pada penelitian kali ini digunakan tiga jenis isolat bakteri dari Lahar Gunung Semeru yaitu Uncultured bacterium clone 1, Uncultured Pseudomonas sp. clone 034571 018, dan Bacterium strain BS2049 untuk proses reduksi Fe(III) menjadi Fe(II) dengan metode spektrofotometri UV-Vis. Fe(II) hasil aktivitas bakteri kemudian dibuat menjadi senyawa kompleks dengan penambahan 1,10-Fenantrolin menghasilkan larutan berwarna jingga-merah. Berlangsungnya bioreduksi Fe(III) menjadi Fe(II) oleh tiga jenis isolat tersebut diamati pada varian temperatur 20°C dan 37°C. Hasil dari penelitian ini berupa persen perolehan kembali besi hasil reduksi setelah inkubasi selama 72 jam. Uncultured bacterium clone 1 mampu mencapai persen perolehan kembali sebesar 91,149% pada temperatur 20°C dan 89,742% pada temperatur 37°C. Uncultured Pseudomonas sp. clone 034571 018 mampu mencapai persen perolehan kembali sebesar 90,425% pada temperatur 20°C dan 93,799% pada temperatur 37°C. Sedangkan Bacterium strain BS2049 mampu mencapai persen perolehan kembali sebesar 91,232% pada temperatur 20°C dan 93,158% pada temperatur 37°C. Aktivitas reduksi besi dari Uncultured bacterium clone 1 lebih optimal pada temperatur 20°C. Sedangkan aktivitas reduksi besi dari Uncultured Pseudomonas sp. clone 034571 018 dan Bacterium strain BS2049 optimal pada temperatur 37°C.
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Iron is the fourth most abundant elements in the earth's crust. This abundance of iron can be utilized by organisms in their metabolic processes. But in excess, iron can cause toxic effects. Industrial development has increased the number of electroplating industries that produce various heavy metal wastes. One of which is iron. Therefore, environmental remediation are needed to maintain the abundance of iron in nature so it will not endanger organisms and the ecosystem. One of the remediation methods that is environmentally friendly and safe to implement is bioremediation by utilizing the activity of microorganisms. In this study, three types of bacterial isolates from Mount Semeru mud flow, Uncultured bacterium clone 1, Uncultured Pseudomonas sp. clone 034571 018, and Bacterium strain BS2049 were used for the reduction of Fe(III) to Fe(II) using UV-Vis spectrophotometer. Fe(II) that produced from bacterial activity was then made into a complex compound by adding 1,10-phenanthroline to produce an orange-red solution. The bioreduction of Fe(III) to Fe(II) by the three isolates was observed at temperature variations of 20°C and 37°C. The results of this study were the percent recovery of reduced iron after incubation for 72 hours. The percent recovery achieved from the activity of Uncultured bacterium clone 1 is up to 91.149% at 20°C and 89.742% at 37°C. The activity of Uncultured Pseudomonas sp. clone 034571 018 was able to achieve percent recovery up to 90.425% at 20°C and 93.799% at 37°C. Meanwhile, Bacterium strain BS2049 was able to achieve percent recovery up to 91.232% at 20°C and 93.158% at 37°C. The iron reduction activity of Uncultured bacterium clone 1 was optimum at 20°C. Meanwhile, the iron reduction activity of Uncultured Pseudomonas sp. clone 034571 018 and Bacterium strain BS2049 were optimum at 37°C.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Besi, Bioreduksi, Gunung Semeru, Spektrofotometer UV-Vis, Temperatur, Iron, Bioreduction, Mount Semeru, Spectrophotometer UV-Vis, Temperature
Subjects:	Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry Q Science > QD Chemistry > QD75.2 Chemistry, Analytic Q Science > QR Microbiology > QR74.8 Bacteria
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Ivana Caroline Sinaga
Date Deposited:	15 Feb 2023 02:40
Last Modified:	15 Feb 2023 02:40
URI:	http://repository.its.ac.id/id/eprint/97250

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