Analisis Konsentrasi Optimum Antigen NS1 terhadap Sensitivitas Biosensor Virus Dengue Berbasis CuInS2/ZnS Quantum Dots

Anggraini, Annisa Rahma (2023) Analisis Konsentrasi Optimum Antigen NS1 terhadap Sensitivitas Biosensor Virus Dengue Berbasis CuInS2/ZnS Quantum Dots. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Penyakit DBD meningkat drastis selama dua dekade terakhir sehingga menjadi ancaman serius bagi masyarakat global dan diperkirakan 100-400 juta terjadi infeksi dan angka kematian 2,5% setiap tahunnya. Deteksi dini virus dengue sangat diperlukan untuk mencegah tingkat keparahan dengan penanganan yang cepat dan tepat. Metode antigen NS1 merupakan salah satu alternatif karena dapat mendeteksi pada awal infeksi, mudah, murah, dan sederhana, namun sensitivitasnya rendah. Oleh karena itu, penelitian ini menganalisis biosensor berbasis photoluminescence dengan memanfaatkan material CuInS2 QDs/ZnS yang bersifat non-toxic dan mampu mendeteksi dengue pada awal infeksi untuk memperoleh nilai LOD rendah dan sensitivitas yang tinggi baik pada larutan phosphate buffer maupun human plasma serum. Proses sintesis CuInS2 QDs/ZnS menggunakan metode wet chemical dengan teknik hot injection. Kemudian, dibiokonjugasi dengan streptavidin serta antibodi NS1 dengan konsentrasi 10 dan 30 nM. Hasil suspensi CuInS2/ZnS-Sa-Ab dicampurkan dengan antigen NS1 variasi konsentrasi 220, 260, dan 300 nM dalam larutan PBS dan HPS. Sampel diuji dengan pengujian FTIR, UV-Vis, dan Photoluminescence. Dalam penelitian ini, nilai LoD dan LoQ yang diperoleh sebesar 0.5540 ng/mL dan 1.8466 ng/mL. Range konsentrasi optimum antigen NS1 yang dapat dideteksi biosensor pada CIS/ZnS-Sa-Ab 10 nM adalah 0 hingga 260 nM dengan nilai sensitivitas tertinggi pada konsentrasi 260 nM sebesar 54.78%. Sedangkan pada CIS/ZnS-Sa-Ab 30 nM, range konsentrasi optimumnya antara 220 hingga 300 nM dengan nilai sensitivitas tertinggi pada konsentrasi 300 nM sebesar 63.94%. Respon biosensor CIS/ZnS-Sa-Ab10 dalam kondisi PBS ke HPS mengalami kenaikan sensitivitas atau quenching fluorescence dari 33.20% menjadi 54.78%, dimana hampir dua kali lipatnya dengan kondisi PBS, namun tetap dalam pola penurunan yang sama baik pada PL quenching maupun lifetime. Sedangkan, pada CIS/ZnS-Sa-Ab30 dalam kondisi PBS ke HPS memiliki nilai sensitivitas dari 59.74% menjadi 63.94%, dimana hampir sama dengan kondisi PBS-nya. Selain itu, penurunan intensitas photoluminescence dan lifetime-nya juga memiliki pola yang sama.
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Dengue fever has increased dramatically over the last two decades so that it becomes a serious threat to the global community and an estimated 100-400 million infections occur and a mortality rate of 2.5% each year. Early detection of dengue virus is needed to prevent its severity with prompt and appropriate treatment. The NS1 antigen method is an alternative because it can detect infection early, is easy, inexpensive, and simple, but has low sensitivity. Therefore, this study analyzed a photoluminescence-based biosensor using CuInS2 QDs/ZnS material which is non-toxic and capable of detecting dengue early in infection to obtain low LoD values and high sensitivity in both phosphate buffer solution and human plasma serum. The process of synthesis of CuInS2 QDs/ZnS uses the wet chemical method with the hot injection technique. Then, it was bioconjugated with streptavidin and NS1 antibodies with concentrations of 10 and 30 nM. The CuInS2/ZnS-Sa-Ab suspension was mixed with NS1 antigen with various concentrations of 220, 260, and 300 nM in PBS and HPS solutions. Samples were tested with FTIR, UV-Vis and Photoluminescence tests. In this study, the LoD and LoQ values obtained were 0.5540 ng/mL and 1.8466 ng/mL. The optimum concentration range of NS1 antigen that can be detected by the biosensor on CIS/ZnS-Sa-Ab 10 nM is 0 to 260 nM with the highest sensitivity value at 260 nM of 54.78%. Whereas in CIS/ZnS-Sa-Ab 30 nM, the optimum concentration range is between 220 and 300 nM with the highest sensitivity value at 300 nM concentration of 63.94%. The response of the CIS/ZnS-Sa-Ab10 biosensor under PBS to HPS conditions experienced an increase in sensitivity or quenching fluorescence from 33.20% to 54.78%, which was almost double that under PBS conditions, but remained in the same decreasing pattern both in PL quenching and lifetime. Meanwhile, CIS/ZnS-Sa-Ab30 under PBS to HPS conditions had a sensitivity value of 59.74% to 63.94%, which is almost the same as the PBS condition. In addition, the decrease in photoluminescence intensity and lifetime also has the same pattern.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Biosensor, Limit of Detection, Photoluminescence, Sensitivitas, Quantum Dots
Subjects:	R Medicine > R Medicine (General) T Technology > TP Chemical technology > TP248 Nanogels. Nanoparticles. T Technology > TP Chemical technology > TP248.3 Biochemical engineering. Bioprocess engineering
Divisions:	Faculty of Industrial Technology and Systems Engineering (INDSYS) > Material & Metallurgical Engineering > 28201-(S1) Undergraduate Thesis
Depositing User:	Annisa Rahma Anggraini
Date Deposited:	24 Jul 2023 07:24
Last Modified:	24 Jul 2023 07:24
URI:	http://repository.its.ac.id/id/eprint/99129

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