PENINGKATAN MASA SIMPAN AKTIVATOR KOMPOS MELALUI VARIASI SUMBER NITROGEN

BARSELIA, ARIDA WAHYU (2016) PENINGKATAN MASA SIMPAN AKTIVATOR KOMPOS MELALUI VARIASI SUMBER NITROGEN. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Kompos merupakan hasil degradasi bahan organik secara aerob maupun anaerob dengan memanfaatkan potensi mikroorganisme sebagai aktivator kompos. Kualitas aktivator kompos sangat tergantung dari kecepatan degradasi yang dimiliki dan lama masa simpannya. Medium pertumbuhan berpengaruh penting terhadap masa simpan aktivator kompos karena untuk mengomptimalkan viabilitas sel dalam waktu yang lama. Salah satu komponen medium pertumbuhan adalah nitrogen berupa protein atau senyawa peptida yang dapat memengaruhi masa simpan (life span) aktivator kompos. Kompleksitas struktur protein menyebabkan pemanfaatan nitrogen untuk pertumbuhan juga berbeda, yang salah satunya berpengaruh terhadap masa simpan aktivator kompos. Tujuan penelitian ini adalah mengetahui pengaruh beberapa sumber nitrogen antara lain yeast extract, pepton, susu skim, dan rumput laut, yang berpotensi untuk meningkatan masa simpan aktivator kompos. Konsorsium aktivator kompos berupa Aspergillus niger dan Rhizobium sp. dengan perbandingan 1:1 dikultur pada medium variasi sumber nitrogen dengan konsentrasi yang berbeda yaitu 2,5% dan 1,25% dengan 3 kali pengulangan. Jumlah koloni dihitung dengan metode TPC (Total Plate Agar), Kadar protein terlarut pada medium kultur dianalisis dengan metode Bradford. Hasil kultur aktivator kompos diaplikasikan untuk pembuatan kompos. Data dianalisis secara deskriptif berupa kurva pertumbuhan koloni, kemiringan kurva pertumbuhan aktivator kompos,kadar protein terlarut, suhu kompos, dan tabel analisis rasio C/N. Hasil penelitian didapatkan bahwa medium rumput laut konsentrasi 1,25% mampu meningkatkan masa simpan aktivator kompos karena hasil kemiringan kurva pertumbuhan relatif rendah sehingga diasumsikan jumlah sel yang cenderung stabil. Sedangkan nilai rasio C/N terbaik adalah aktivator kompos pada medium rumput laut konsentrasi 1,25% karena nilai yang didapatkan sebesar 14,15/1. Sehingga medium rumput laut konsentrasi 1,25% dapat dimanfaatkan untuk meningkatkan masa simpan aktivator kompos. ============================================================ Compost is an aerobic or anaerobic organic material degradation using microorganism as compost activator. Microorganism has hydrolisis enzim to accelerate composting process for degrade organic material. Quality of compost activator depend on organic degradation time and the life span of compost activator. The life span of compost activator was influenced of growth medium to optimize cell viability. Main component of growth medium is protein which contain nitrogen sources and has role for compost activator’s life span. Complexity of protein structure causes difference time to utilize it, thus this mechanism can enhance activator compost life span. Therefore, enhacing compost activator can be optimized by varying nitrogen sources, such as pepton, yeast extract, skim milk, and seaweed. This research aimed at ascertaining nitrogen sources has role to influence life span of compost activator and the activity of compost activator can be improve by composting process. The ratio of compost activator consortium is 1:1 consist of Aspergillus niger and Rhizobium sp. Consortium is cultured into variation nitrogen sources medium by different concentration, 2,5% and 1,25% and repeated in 3 times. Total colony is measured by TPC method in 6 dillutions. The protein content was measured by using Bradford method. Then the activator compost is applied for making compost from leaves waste as the organic material. The degradation activity of compost activator is analyzed by C/N ratio. The data of this research is analyzed in descriptive by arrange colony growth curve, slope of activator compost growth, protein content, temperature of compost, and table of C/N ratio. Based on the research, the best medium for enhancing activator compost life span is seaweed concentration 1,25% because the slope analyze was low than the other and it was assumed that cell total was stable based on the death phase was low. Besides, seaweed was easily to maintain for making medium growth of compost activator and cheap. Then, the best activator compost for C/N ratio was determined in seaweed medium with the result was 14,15/1.

Item Type: Thesis (Undergraduate)
Additional Information: RSBi 631.86 Bar p
Uncontrolled Keywords: aktivator kompos, masa simpan, variasi sumber nitrogen
Subjects: Q Science > QH Biology > QH301 Biology
Divisions: Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User: Mrs Anis Wulandari
Date Deposited: 09 Jan 2017 07:47
Last Modified: 26 Dec 2018 06:49
URI: https://repository.its.ac.id/id/eprint/1414

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