Pertiwi, Lintang (2017) Uji Sitotoksisitas Empat Trisindolina Terhadap Kanker Payudara MCF-7 Cell Line. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Kanker adalah penyakit genetik yang disebabkan karena adanya mutasi DNA. Pada tahun 2014, kanker pada wanita dengan prevelensi tertinggi adalah kanker payudara (21,4%). Doksorubisin, merupakan salah satu agen kemoterapi kanker payudara. Namun, penggunaannya dapat memberikan efek kardiotoksik, mielosupresi dan mucositis pada sel normal. Karena itu,perlu adanya senyawa alternatif Doksorubisin, salah satu yang potensial adalah trisindolina. Penelitian ini dilakukan untuk menguji efek sitotoksisitas empat trisindolina pada kanker payudara MCF (Michigan Cancer Foundation)-7 cell line. Uji sitotoksisitas senyawa dilakukan dengan menggunakan metode uji MTT (3-(4,5-dimetiltiazol-2-yl)-2,5-dipeniltetrazolium bromid). Kultur sel diberi perlakuan senyawa dengan 7 variasi konsentrasi (200; 100; 50; 25; 12,5; 6,25; 3,125). Sebagai kontrol, digunakan perlakuan Doksorubisin. Optical density diukur dengan microplate reader. Hasilnya dianalisa menggunakan regresi polinomial dan ditampilkan dalam bentuk nilai IC50. Hasilnya, nilai IC50 keempat senyawa uji berturut-turut adalah 2,059 ; tidak memiliki aktivitas ; 3,9759 ; 15,46 μM. Perbedaan nilai IC50 tersebut disebabkan oleh penambahan gugus tertentu pada struktur senyawa uji. ================================================================= Cancer is a genetic disease cause by DNA mutation. In 2014, breast cancer become the highest case of cancer on woman (21,4% ). Doxorubicin, is one of the common chemotherapy agent for breast cancer . But, the use of doxorubicin brings the side effect like cardiotoxicity, myelosupression and mucoitis on the normal cell. To eliminate this negative effect, people need to find alter native compound for chemotherapy agent. One of the most potential compound is trisindoline . The aim of this research is to test the cytotoxicity effect of four trisindoline against breast cancer MCF (Michigan Cancer Foundation) -7 cell line. This assay use MTT (3-(4,5- dimetiltiazol- 2-yl) -2,5- dipeniltetrazolium bromid) method. Seven series (200; 100; 50; 25; 12,5; 6,25; 3,125) of test compound was added to the cell culture. Doxorubicin was used for a control treatment. The optical density was analyzed using m icroplate reader and the result shown as a value of IC 50 . As a result, value of four trisindoline are 2,059 ; undefined ; 3,9759 ; 15,46 μM. The difference between IC 50 is caused by the adding of specific group on the each compound.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Uncontrolled Keywords: | Kanker, Uji Sitotoksisistas, Uji MTT, Trisindolina, Cancer, Cytotoxicity Assay, MCF-7, MTT Assay, Trisindoline. |
| Subjects: | Q Science > QD Chemistry Q Science > QL Zoology Q Science > QM Human anatomy Q Science > QP Physiology R Medicine > RB Pathology R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica |
| Divisions: | Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | - LINTANG PERTIWI |
| Date Deposited: | 18 Apr 2017 02:21 |
| Last Modified: | 22 Dec 2017 06:57 |
| URI: | https://repository.its.ac.id/id/eprint/3785 |
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