Analisa Asam Lemak Ragi Rhodotorula Graminis NBRC 0190

Thoyyibah, Qurrota A`yun (2016) Analisa Asam Lemak Ragi Rhodotorula Graminis NBRC 0190. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Penelitian berikut mengenai pengidentifikasian asam lemak yang terdapat pada spesies Rhodotorula graminis NBRC 0190 pada National Institute of Technology and Evaluation Jepang. Ragi R. graminis dibiakkan pada media padat dan media cair berupa campuran glukosa, pepton dan ekstrak yeast (1:0,5:0,3). Selanjutnya ragi diinokulasi selama rentang waktu tertentu sesuai data kurva pertumbuhan. Biomassa hasil inokulasi kemudian dibersihkan dan dikeringkan selama 24 jam. Sel kering diambil lipid totalnya dengan larutan kloroform-metanol (2:1). Lipid total selanjutnya ditransesterifikasi agar dapat diketahui kandungan asam lemaknya dengan metode KLT dan KG-SM. Hasil penelitian yang diperoleh menunjukkan bahwa pada plat KLT dengan eluen petroleum eter:dietil eter:etil asetat (3:3:2) terdapat perubahan senyawa lipid menjadi senyawa metil ester setelah mengalami reaksi transesterifikasi serta adanya delapan asam lemak yang muncul pada kromatogram KG. Kedelapan asam lemak tersebut yaitu metil heksadekanoat, asam z-7- heksadekenoat; asam n-heksadekanoat; metil oktadek-9-enoat, metil 14-metil-heptadekanoat, asam oleat, asam oktadek-9-enoat; dan bis(2-etilheksil) adipat yang telah umum ditemukan pada R. graminis lainnya ================================================================================================================== The following studies regarding the identification of fatty acids contained in the species Rhodotorula graminis NBRC 0190 Laboratory at the National Institute of Technology and Evaluation Japan. Yeast R. graminis cultured on solid media and liquid media in the form of a mixture of glucose, peptone and yeast extract (1:0.5:0.3). Furthermore, the yeast inoculated during a certain time span for which data growth curve. Biomass results of inoculation is then cleaned and dried for 24 hours. Dry cell taken total lipid with a solution of chloroform-methanol (2: 1). The next total lipid transesterified in order to know the fatty acid content by using TLC and GC-MS. The results obtained show that the TLC plate with eluent petroleum ether:diethyl ether:ethyl acetate (3:3:2) there are changes in lipid compound into methyl ester compound after a transesterification reaction as well as the presence of eight fatty acid that appears on the chromatogram GC. These 8th fatty acids, namely hexadecanoic acid, methyl esters; z-7-hexadecenoic acid, n-hexadecanoic acid, 9- octadecanoic acid, methyl esters; heptadecanoic acid, 14-methyl-, methyl esters; octadec-9-enoic acid; and hexanedioic acid, bis(2- ethylhexyl) ester which is commonly found on the others R. graminis.

Item Type: Thesis (Undergraduate)
Additional Information: RSKi 579.562 Tho a
Uncontrolled Keywords: Asam lemak, klasifikasi mikroorganisme,Rhodotorula graminis
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Mathematics and Science > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User: Users 13 not found.
Date Deposited: 19 Jun 2017 03:06
Last Modified: 27 Dec 2018 07:59
URI: https://repository.its.ac.id/id/eprint/41729

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