., Cecilia and Kristian, Wahyu Dwi (2017) Pengaruh Siklus Pencahayaan terhadap Pertumbuhan dan Produktivitas Lipid Mikroalga Botryococcus braunii Termutasi UV-B dan Alami. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Terjadinya krisis energi menyebabkan meningkatnya penggunaan sumber energi alternatif sebagai pengganti sumber energi fosil yang tidak dapat diperbaharui. Salah satunya pembuatan biodiesel sebagai pengganti solar. Mikroalga merupakan sumber energi terbarukan yang layak untuk dikembangkan. Botryococcus braunii merupakan jenis mikroalga yang saat ini banyak diujikan karena kandungan lipidnya yang cukup tinggi. Penambahan kadar CO2 dan siklus pencahayaan dilakukan untuk melihat pengaruhnya terhadap pertumbuhan dan produktivitas lipid mikroalga.
Tahap pertama adalah mengembangbiakkan mikroalga Botryococcus braunii alami dan termutasi UV-B selama 3 hari dengan pemberian nutrient setiap 12 jam. Tahap selanjutnya adalah penambahan CO2 dengan flowrate 15 mL/menit disertai siklus pencahayaan dengan variabel (terang : gelap) 12 jam : 12 jam, 16 jam : 8 jam, dan 24 jam : 0 jam. Pertumbuhan mikroalga dihitung dengan menggunakan hemasitometer dan pengecekan Optical Density (OD) pada panjang gelombang 680 nm setiap 24 jam. Untuk mendapatkan lipid digunakan ekstraksi menggunakan n-heksana.
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Energy crisis leads to the use of alternative energy sources as replacement for non-renewable energy sources. One of them is biodiesel manufacture as a substitution of diesel fuel. Microalgae are a viable source of renewable energy to be developed.
Botryococcus braunii is a microalgae that currently being tested for its ability as diesel fuel source because of its high lipid content. Enhanced of CO2 and light cycle were done to check the effect on microalgae growth rate and lipid productivity.
First stage is to breed natural and mutant microalgae Botryococcus braunii cultivation for 3 days with nutrient adding once every 12 hours. The next stage is CO2 addition with flowrate 15 mL/min and light cycle ratio (light : dark) varied to 3 variables = 12 hours : 12 hours, 16 hours : 8 hours, and 24 hours : 0 hours. Microalgae growth is calculated with haemacytometer and monitored by measuring the Optical Density (OD) at 680 nm wavelength every 24 hours. Extraction of lipid using n-hexane to obtain the lipid.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSK Cec p-1 |
| Uncontrolled Keywords: | Botryococcus braunii, CO2, microalgae, mutation, UV-B |
| Subjects: | Q Science > QK Botany > QK568 Microalgae T Technology > TP Chemical technology > TP156 Crystallization. Extraction (Chemistry). Fermentation. Distillation. Emulsions. |
| Divisions: | Faculty of Industrial Technology > Chemical Engineering > 24201-(S1) Undergraduate Thesis |
| Depositing User: | Wahyu Dwi Kristian |
| Date Deposited: | 15 Nov 2017 08:19 |
| Last Modified: | 05 Mar 2019 08:01 |
| URI: | http://repository.its.ac.id/id/eprint/43400 |
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