Permanasari, Yunita (2015) Pengaruh asam salisilat dan fenilalanin terhadap kandungan total asam fenol pada kultur suspensi sel moringa oleifera lam. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Moringa oleifera merupakan tanaman yang berpotensi
tinggi untuk dikembangkan dalam sektor industri seperti
industri farmasi karena kandungan senyawa fenolnya yang
tinggi. Tujuan dari penelitian ini untuk mengetahui pengaruh
asam salisilat sebagai elisitor dan phenilalanin sebagai
prekursor terhadap kandungan total asam fenol pada kultur
suspensi sel M. oleifera.
Rancangan penelitian yang digunakan adalah RAL
(rancangan acak lengkap) dengan ulangan sebanyak 3 kali.
Parameter uji yang dilakukan adalah estimasi senyawa total
asam fenol menggunakan spektrofotometer UV-Vis dan berat
kering hasil perlakuan asam salisilat dan fenilalanin. Hasil
dianalisa menggunakan anova one way dan uji Tukey.
Hasil penelitian menunjukkan variasi konsentrasi
asam salisilat dan fenilalanin berpengaruh terhadap
peningkatan kandungan total asam fenol pada kultur
suspensi sel M. oleifera Total asam fenol tertinggi
terdapat pada asam salisilat 1 ppm 1,6067± 0,458 ppm
dan fenilalanin 5 ppm 1,60 ± 0,37 ppm.
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Moringa oleifera is a plant that has high potential to
be developed in industrial sectors such as the pharmaceutical
industry because of the high content of phenol compounds.
The purpose of this study was to determine the effect of
salicylic acid as elicitor and phenylalanine as a precursor to
the total content of phenolic acids in cell suspension cultures
of M. oleifera.
The research design used was RAL (completely
randomized design) with 3 replications. Test parameters the
estimation of the total phenolic acids compounds using UVVis
spectrophotometer and the dry weight of salicylic acid and
phenylalanine treatment. The result were analyzed by oneway
ANOVA and Tukey test.
The results showed that varying concentration of
salicylic acid and phenylalanine affect the increase of the total
content of phenolic acids in cell suspension cultures of M.
oleifera. The highest total phenolic acids contained at
concentration of salicylic acid 1 ppm was 1.6067 ± 0.458 ppm
and phenylalanine 5 ppm was 1,60 ± 0.37 ppm
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSBi 583 64 Per p |
| Uncontrolled Keywords: | asam fenol ,asam salisilat, fenilalanin, M. oleifera |
| Subjects: | Q Science > QH Biology > QH75 Nature conservation. Landscape protection. Biodiversity conservation. Endangered species and ecosystems (General). Habitat conservation. Ecosystem management. Conservation biology |
| Divisions: | Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | - Taufiq Rahmanu |
| Date Deposited: | 21 Nov 2019 07:15 |
| Last Modified: | 21 Nov 2019 07:15 |
| URI: | http://repository.its.ac.id/id/eprint/71950 |
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