Uji potensi khamir yang diisolasi dari kawasan mangrove Pantai Kenjeran Surabaya dalam mendegradasi selulosa

Emilisari, Martha (2015) Uji potensi khamir yang diisolasi dari kawasan mangrove Pantai Kenjeran Surabaya dalam mendegradasi selulosa. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Molekul selulosa merupakan polimer linier, bersifat kristalin dan tidak mudah larut. Hal ini membuat selulosa tidak mudah didegradasi secara kimia dan mekanis, sehingga dibutuhkan kajian tentang penanganan degradasi selulosa secara biologis dengan bantuan mikroorganisme. Salah satu mikroorganisme potensial yang memiliki kemampuan dalam mendegradasi selulosa adalah khamir. Penelitian ini bertujuan untuk mengetahui genus khamir hasil isolasi dari mangrove pantai Kenjeran Surabaya yang berpotensi dalam mendegradasi selulosa, untuk mengetahui indeks hidrolisis selulosa tertinggi dari khamir, serta kadar gula reduksi tertinggi dari khamir. Isolat khamir yang diperoleh diidentifikasi hingga tingkat genus. Uji potensi selulolitik khamir secara kualitatif dilakukan dengan menggunakan medium CMC. Sementara, uji potensi secara kuantitatif dilakukan dengan pengukuran kadar gula reduksi dengan metode DNS pada isolat terbaik dari hasil uji kualitatif. Hasil penelitian diperoleh lima isolat dengan kode K 1.2, K 2.2, K 2.3, K 2.4, dan K 4.2. Isolat-isolat tersebut berpotensi dalam mendegradasi selulosa. Nilai indeks selulolitik tertinggi dicapai oleh isolat K 1.2 dengan nilai sebesar 0,725. Kadar gula reduksi tertinggi yang dicapai oleh isolat K 1.2 adalah 0,190ppm pada hari ketiga. ========== Cellulose is a linear polymer molecules, which is crystalline and easily soluble. This makes the cellulose is not easily degraded chemically and mechanically, so a study on the cellulose degradation by biological treatment using microorganisms is extremely needed. One of microorganisms which have the ability to degrade cellulose is yeast. This study is not only aims to determine the potential genus of yeasts to degrade cellulose isolated from the mangrove areas of Kenjeran Surabaya, but also to determine the highest index of yeast on cellulose hydrolysis, and the highest sugar reduction level of yeast. Yeast isolates were identified to the genus level. Test potential cellulolytic yeasts qualitatively done using CMC medium. Meanwhile, the potential for quantitative test is done by measuring the levels of reducing sugars with the DNS method on the best isolates from qualitative test results. The results were obtained five isolates with the code K 1.2, K 2.2, K 2.3, K 2.4 and K 4.2. Isolates have the potential to degrade cellulose. The highest index value cellulolitic achieved by isolates K 1.2 with a value of 0.725. The highest reducing sugar levels are achieved by isolates K 1.2 is 0,190 ppm on the third day.

Item Type: Thesis (Undergraduate)
Additional Information: RSBi 572.566 82 Emi u 3100015061194
Uncontrolled Keywords: khamir, selulosa, degradasi, gula reduksi, yeast, cellulose, degradation, reducing sugar
Subjects: Q Science > QD Chemistry > QD320 Cellulose. Hydrolysis
Divisions: Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User: - Davi Wah
Date Deposited: 09 Dec 2019 08:03
Last Modified: 09 Dec 2019 08:03
URI: https://repository.its.ac.id/id/eprint/72264

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